Carboxylicivirga marinus sp. nov., Isolated From Marine Sediment, and Genome Insight of the Genus Carboxylicivirga

A Gram-stain-negative, facultatively aerobic, beige and slender bacterium, designed N1Y132(T), was isolated from a sediment sample taken from coastal zone of Weihai, China (37 degrees 34 ' 31.44 '' N, 122 degrees 9 ' 15 '' E). Growth occurred at 15-33 degrees C (optimal 25-28 degrees C), at pH 6.5-8.5 (optimal pH 7.5), in 0-9% (w/v) NaCl (optimal 2.0-2.5%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain N1Y132(T) showed the highest sequence similarity (97.9%) with Carboxylicivirga taeanensis JCM 19490(T), followed by C. sediminis JR1(T) (97.4%), C. mesophila JCM 18290(T) (96.4%) and C. linearis FB218(T) (95.1%). Thus, strain N1Y132(T) was a member of the genus Carboxylicivirga. The average nucleotide identity (ANI) values between strain N1Y132(T) and C. sediminis JR1(T), C. linearis FB218(T) and C. mesophila JCM 18290(T) were 74.8%, 71.1%, and 74.9%, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was MK-7 and the major fatty acids (> 5.0%) included iso-C-15(:)0, anteiso-C-15(:)0, iso-C-13(:)0, iso-C-14(:)0, iso-C-15(:)0 3-OH, and C-15(:)1 omega 6c. The polar lipids were consisted of a phosphatidylethanolamine, two phosphoaminolipids and six unidentified lipids. The DNA G + C content was 38.1 mol%. Based on the evidence presented in this study, strain N1Y132(T) represents a novel species of the genus Carboxylicivirga, for which the name Carboxylicivirga marinus sp. nov. is proposed. The type strain is N1Y132(T) (= KCTC 72934(T) = MCCC 1H00431(T)).