Barcoded microbial system for high-resolution object provenance

被引：33

作者：

Qian, Jason ^{[1
,2
,3
]}

Lu, Zhi-xiang ^{[1
,2
]}

Mancuso, Christopher P. ^{[4
,5
]}

Jhuang, Han-Ying ^{[1
]}

Barajas-Ornelas, Rocio del Carmen ^{[6
]}

Boswell, Sarah A. ^{[1
,2
]}

Ramirez-Guadiana, Fernando H. ^{[6
]}

Jones, Victoria ^{[1
,7
,10
]}

Sonti, Akhila ^{[4
,5
]}

Sedlack, Kole ^{[4
,5
,11
]}

Artzi, Lior ^{[6
]}

Jung, Giyoung ^{[8
]}

Arammash, Mohammad ^{[1
]}

Pettit, Mary E. ^{[1
]}

Melfi, Michael ^{[1
]}

Lyon, Lorena ^{[1
]}

Owen, Sian V. ^{[7
]}

Baym, Michael ^{[2
,7
]}

Khalil, Ahmad S. ^{[4
,5
,9
]}

Silver, Pamela A. ^{[1
,9
]}

Rudner, David Z. ^{[6
]}

Springer, Michael ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA

[2] Harvard Med Sch, Lab Syst Pharmacol, Boston, MA 02115 USA

[3] Harvard Med Sch, Biol & Biomed Sci Program, Boston, MA 02115 USA

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Boston Univ, Biol Design Ctr, Boston, MA 02215 USA

[6] Harvard Med Sch, Dept Microbiol & Immunol, Boston, MA 02115 USA

[7] Harvard Med Sch, Dept Biomed Informat, Boston, MA 02115 USA

[8] MIT, Synthet Biol Ctr, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[9] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[10] Quanterix, Billerica, MA 01821 USA

[11] Univ Illinois, Coll Med, Rockford, IL 61107 USA

来源：

SCIENCE | 2020年 / 368卷 / 6495期

关键词：

BACILLUS-THURINGIENSIS; TRACKING;

D O I：

10.1126/science.aba5584

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment scalability, rapid and facile decoding, and biocontainment Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating it implementation in a wide range of applications.

引用

页码：1135 / +

页数：47

共 50 条

[41] Hierarchical object detection for very high-resolution satellite images
Wu, Zhi-Ze
Wang, Xiao-Feng
Zou, Le
Xu, Li-Xiang
Li, Xin-Lu
Weise, Thomas
APPLIED SOFT COMPUTING, 2021, 113
[42] Toward object alphabet augmentation for object detection in very high-resolution satellite images
Laban, Noureldin
Abdellatif, Bassam
Ebeid, Hala M.
Shedeed, Howida A.
Tolba, Mohamed F.
CONCURRENCY AND COMPUTATION-PRACTICE & EXPERIENCE, 2022, 34 (06):
[43] HIGH-BRIGHTNESS AND HIGH-RESOLUTION RHEED SYSTEM
VANGORKUM, AA
SMITS, MRT
LARSEN, PK
RAUE, R
REVIEW OF SCIENTIFIC INSTRUMENTS, 1989, 60 (09): : 2940 - 2944
[44] Small object classification performance of high-resolution imaging sonars as a function of image resolution
Manning, R
OCEANS 2002 MTS/IEEE CONFERENCE & EXHIBITION, VOLS 1-4, CONFERENCE PROCEEDINGS, 2002, : 2156 - 2163
[45] Quantitative High-Resolution Imaging of Live Microbial Cells at High Hydrostatic Pressure
Bourges, Anais C.
Lazarev, Alexander
Declerck, Nathalie
Rogers, Karyn L.
Royer, Catherine A.
BIOPHYSICAL JOURNAL, 2020, 118 (11) : 2670 - 2679
[46] HIGH-RESOLUTION MULTIWIRE PROPORTIONAL CHAMBER SYSTEM
FRIEZE, W
DHAWAN, S
DISCO, AA
FAJARDO, L
MAJKA, R
MARX, JN
NEMETHY, P
SANDWEISS, J
SLAUGHTER, AJ
NUCLEAR INSTRUMENTS & METHODS, 1976, 136 (01): : 93 - 97
[47] HIGH-RESOLUTION FLOW ZONAL CENTRIFUGE SYSTEM
MCALEER, WJ
HURNI, W
WASMUTH, E
HILLEMAN, MR
BIOTECHNOLOGY AND BIOENGINEERING, 1979, 21 (02) : 317 - 321
[48] TIMING SYSTEM FOR HIGH-RESOLUTION TIME SPECTROSCOPY
BIALKOWSKI, J
MOSZYNSKI, M
NUCLEAR INSTRUMENTS & METHODS, 1972, 105 (01): : 51 - +
[49] A scalable, high-resolution tiled display system
Kang, Yong-Bin
IMECS 2007: International Multiconference of Engineers and Computer Scientists, Vols I and II, 2007, : 2009 - 2013
[50] THE DEVELOPMENT OF A HIGH-RESOLUTION SPACECRAFT TV SYSTEM
GARLOW, RK
JOURNAL OF THE SMPTE-SOCIETY OF MOTION PICTURE AND TELEVISION ENGINEERS, 1965, 74 (02): : 134 - 134

← 1 2 3 4 5 →