Barcoded microbial system for high-resolution object provenance

被引：33

作者：

Qian, Jason ^{[1
,2
,3
]}

Lu, Zhi-xiang ^{[1
,2
]}

Mancuso, Christopher P. ^{[4
,5
]}

Jhuang, Han-Ying ^{[1
]}

Barajas-Ornelas, Rocio del Carmen ^{[6
]}

Boswell, Sarah A. ^{[1
,2
]}

Ramirez-Guadiana, Fernando H. ^{[6
]}

Jones, Victoria ^{[1
,7
,10
]}

Sonti, Akhila ^{[4
,5
]}

Sedlack, Kole ^{[4
,5
,11
]}

Artzi, Lior ^{[6
]}

Jung, Giyoung ^{[8
]}

Arammash, Mohammad ^{[1
]}

Pettit, Mary E. ^{[1
]}

Melfi, Michael ^{[1
]}

Lyon, Lorena ^{[1
]}

Owen, Sian V. ^{[7
]}

Baym, Michael ^{[2
,7
]}

Khalil, Ahmad S. ^{[4
,5
,9
]}

Silver, Pamela A. ^{[1
,9
]}

Rudner, David Z. ^{[6
]}

Springer, Michael ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA

[2] Harvard Med Sch, Lab Syst Pharmacol, Boston, MA 02115 USA

[3] Harvard Med Sch, Biol & Biomed Sci Program, Boston, MA 02115 USA

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Boston Univ, Biol Design Ctr, Boston, MA 02215 USA

[6] Harvard Med Sch, Dept Microbiol & Immunol, Boston, MA 02115 USA

[7] Harvard Med Sch, Dept Biomed Informat, Boston, MA 02115 USA

[8] MIT, Synthet Biol Ctr, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[9] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[10] Quanterix, Billerica, MA 01821 USA

[11] Univ Illinois, Coll Med, Rockford, IL 61107 USA

来源：

SCIENCE | 2020年 / 368卷 / 6495期

关键词：

BACILLUS-THURINGIENSIS; TRACKING;

D O I：

10.1126/science.aba5584

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment scalability, rapid and facile decoding, and biocontainment Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating it implementation in a wide range of applications.

引用

页码：1135 / +

页数：47

共 50 条

[21] A HIGH-RESOLUTION SYSTEM FOR GRADIENT ANALYSIS
MORTON, BE
HIRSCH, CA
ANALYTICAL BIOCHEMISTRY, 1970, 34 (02) : 544 - &
[22] EVALUATION OF A HIGH-RESOLUTION ELECTROPHORESIS SYSTEM
SUN, T
CHAN, S
GROSS, S
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1977, 67 (03) : 247 - 250
[23] SUPERCONDUCTING ELECTRONOPTICAL SYSTEM WITH HIGH-RESOLUTION
DIETRICH, I
LEFRANC, G
WEYL, R
ZERBST, H
OPTIK, 1973, 38 (04): : 449 - 453
[24] High-resolution imaging of the musculoskeletal system
Erickson, SJ
RADIOLOGY, 1997, 205 (03) : 593 - 618
[25] A system for high-resolution zooplankton imaging
Samson, S
Hopkins, T
Remsen, A
Langebrake, L
Sutton, T
Patten, J
IEEE JOURNAL OF OCEANIC ENGINEERING, 2001, 26 (04) : 671 - 676
[26] Galileo high-resolution encoder system
Mancini, D
Cascone, E
Schipani, P
TELESCOPE CONTROL SYSTEMS II, 1997, 3112 : 328 - 334
[27] A System for High-Resolution Topology Optimization
Wu, Jun
Dick, Christian
Westermann, Ruediger
IEEE TRANSACTIONS ON VISUALIZATION AND COMPUTER GRAPHICS, 2016, 22 (03) : 1195 - 1208
[28] A high-resolution fluid imaging system
Lindsay, S
Zhu, J
Hudson, J
AMERICAN LABORATORY, 1997, 29 (22) : 15 - &
[29] HIGH-RESOLUTION OPTICAL RANGING SYSTEM
PARK, H
CHODOROW, M
KOMPFNER, R
APPLIED OPTICS, 1981, 20 (14): : 2389 - 2394
[30] HIGH-RESOLUTION CATHODOLUMINESCENCE ANALYSIS SYSTEM
DAVIDSON, SM
RASUL, A
JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS, 1977, 10 (01): : 43 - 46

← 1 2 3 4 5 →