Barcoded microbial system for high-resolution object provenance

被引：33

作者：

Qian, Jason ^{[1
,2
,3
]}

Lu, Zhi-xiang ^{[1
,2
]}

Mancuso, Christopher P. ^{[4
,5
]}

Jhuang, Han-Ying ^{[1
]}

Barajas-Ornelas, Rocio del Carmen ^{[6
]}

Boswell, Sarah A. ^{[1
,2
]}

Ramirez-Guadiana, Fernando H. ^{[6
]}

Jones, Victoria ^{[1
,7
,10
]}

Sonti, Akhila ^{[4
,5
]}

Sedlack, Kole ^{[4
,5
,11
]}

Artzi, Lior ^{[6
]}

Jung, Giyoung ^{[8
]}

Arammash, Mohammad ^{[1
]}

Pettit, Mary E. ^{[1
]}

Melfi, Michael ^{[1
]}

Lyon, Lorena ^{[1
]}

Owen, Sian V. ^{[7
]}

Baym, Michael ^{[2
,7
]}

Khalil, Ahmad S. ^{[4
,5
,9
]}

Silver, Pamela A. ^{[1
,9
]}

Rudner, David Z. ^{[6
]}

Springer, Michael ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA

[2] Harvard Med Sch, Lab Syst Pharmacol, Boston, MA 02115 USA

[3] Harvard Med Sch, Biol & Biomed Sci Program, Boston, MA 02115 USA

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Boston Univ, Biol Design Ctr, Boston, MA 02215 USA

[6] Harvard Med Sch, Dept Microbiol & Immunol, Boston, MA 02115 USA

[7] Harvard Med Sch, Dept Biomed Informat, Boston, MA 02115 USA

[8] MIT, Synthet Biol Ctr, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[9] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[10] Quanterix, Billerica, MA 01821 USA

[11] Univ Illinois, Coll Med, Rockford, IL 61107 USA

来源：

SCIENCE | 2020年 / 368卷 / 6495期

关键词：

BACILLUS-THURINGIENSIS; TRACKING;

D O I：

10.1126/science.aba5584

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment scalability, rapid and facile decoding, and biocontainment Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating it implementation in a wide range of applications.

引用

页码：1135 / +

页数：47

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