A Sanger sequencing protocol for SARS-CoV-2 S-gene

被引：16

作者：

Daniels, Rodney S. ^{[1
]}

Harvey, Ruth ^{[1
]}

Ermetal, Burcu ^{[1
]}

Xiang, Zheng ^{[1
]}

Galiano, Monica ^{[1
]}

Adams, Lorin ^{[1
]}

McCauley, John W. ^{[1
]}

机构：

[1] Worldwide Influenza Ctr, WHO Collaborating Ctr Reference & Res Influenza, Francis Crick Inst, London, England

来源：

INFLUENZA AND OTHER RESPIRATORY VIRUSES | 2021年 / 15卷 / 06期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

base-calling accuracy; Sanger sequencing; SARS-CoV-2; S-gene; variant detection; NEUTRALIZING ANTIBODY-ACTIVITY;

D O I：

10.1111/irv.12892

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We describe a Sanger sequencing protocol for SARS-CoV-2 S-gene the Spike (S)-glycoprotein product of which, composed of receptor-binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID-19. The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid "at source" screening for SARS-CoV-2 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis-mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.

引用

页码：707 / 710

页数：4

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