A Sanger sequencing protocol for SARS-CoV-2 S-gene

被引：16
|
作者：
Daniels, Rodney S. [1 ]
Harvey, Ruth [1 ]
Ermetal, Burcu [1 ]
Xiang, Zheng [1 ]
Galiano, Monica [1 ]
Adams, Lorin [1 ]
McCauley, John W. [1 ]
机构：
[1] Worldwide Influenza Ctr, WHO Collaborating Ctr Reference & Res Influenza, Francis Crick Inst, London, England
来源：
INFLUENZA AND OTHER RESPIRATORY VIRUSES | 2021年 / 15卷 / 06期
基金：
英国惠康基金; 英国医学研究理事会;
关键词：
base-calling accuracy; Sanger sequencing; SARS-CoV-2; S-gene; variant detection; NEUTRALIZING ANTIBODY-ACTIVITY;
D O I：
10.1111/irv.12892
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We describe a Sanger sequencing protocol for SARS-CoV-2 S-gene the Spike (S)-glycoprotein product of which, composed of receptor-binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID-19. The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid "at source" screening for SARS-CoV-2 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis-mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.
引用
收藏
页码：707 / 710
页数：4
相关论文
共 50 条
  • [21] A Routine Sanger Sequencing Target Specific Mutation Assay for SARS-CoV-2 Variants of Concern and Interest
    Lee, Sin Hang
    VIRUSES-BASEL, 2021, 13 (12):
  • [22] Coding-Complete Genome Sequence of SARS-CoV-2 Isolate from Bangladesh by Sanger Sequencing
    Moniruzzaman, M.
    Hossain, Mohammad Uzzal
    Islam, M. Nazrul
    Rahman, M. Hadisur
    Ahmed, Irfan
    Rahman, Tahia Anan
    Bhattacharjee, Arittra
    Amin, M. Ruhul
    Rashed, Asif
    Keya, Chaman Ara
    Das, Keshob Chandra
    Salimullah, M.
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2020, 9 (28):
  • [23] Development of an efficient Sanger sequencing-based assay for detecting SARS-CoV-2 spike mutations
    Lim, Ho Jae
    Park, Min Young
    Jung, Hye Soo
    Kwon, Youngjin
    Kim, Inhee
    Kim, Dong Kwan
    Yu, Nae
    Sung, Nackmoon
    Lee, Sun-Hwa
    Park, Jung Eun
    Yang, Yong-Jin
    PLOS ONE, 2021, 16 (12):
  • [24] Enhancing the epidemiological surveillance of SARS-CoV-2 using Sanger sequencing to identify circulating variants and recombinants
    Silva, Thais
    Oliveira, Eneida
    Oliveira, Alana
    Menezes, Andre
    Jeremias, Wander de Jesus
    Grenfell, Rafaella F. Q.
    Monte-Neto, Rubens Lima do
    Pascoal-Xavier, Marcelo A.
    Campos, Marco A.
    Fernandes, Gabriel
    Alves, Pedro
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2024, 55 (03) : 2085 - 2099
  • [25] High concordance of the TaqMan SARS-CoV-2 mutation panel and spike gene sequencing for the detection of SARS-COV-2 Variants Of Concern
    Tagnouokam-Ngoupo, Paul Alain
    Monamele, Chavely Gwladys
    Sadeuh-Mba, Serge Alain
    Tchatchueng-Mbougua, Jules Brice
    Longla, Estelle Madaha
    Abanda, Ngu Njei
    Ilouga, Vanessa
    Yifomnjou, Moise Henri Moumbeket
    Tejiokem, Mathurin Cyrille
    Eyangoh, Sara
    Njouom, Richard
    JOURNAL OF CLINICAL VIROLOGY PLUS, 2023, 3 (03):
  • [26] Development of multiplex S-gene-targeted RT-PCR for rapid identification of SARS-CoV-2 variants by extended S-gene target failure
    Imaizumi, Yuri
    Ishige, Takayuki
    Fujikawa, Tatsuki
    Miyabe, Akiko
    Murata, Shota
    Kawasaki, Kenji
    Nishimura, Motoi
    Taniguchi, Toshibumi
    Igari, Hidetoshi
    Matsushita, Kazuyuki
    CLINICA CHIMICA ACTA, 2022, 536 : 6 - 11
  • [27] SpikeSeq: A rapid, cost efficient and simple method to identify SARS-CoV-2 variants of concern by Sanger sequencing part of the spike protein gene
    Jorgensen, Tue Sparholt
    Pedersen, Martin Schou
    Blin, Kai
    Kuntke, Franziska
    Salling, Henrik K.
    Marvig, Rasmus L.
    Michaelsen, Thomas Y.
    Albertsen, Mads
    Larsen, Helene
    JOURNAL OF VIROLOGICAL METHODS, 2023, 312
  • [28] Partial N Gene Sequencing for SARS-CoV-2 Verification and Pathway Tracing
    Lee, Sin Hang
    McGrath, Jonathan
    Connolly, Stephen P.
    Lambert, John
    INTERNATIONAL MEDICAL CASE REPORTS JOURNAL, 2021, 14 : 1 - 10
  • [29] A Sanger approach based on overlapping fragments to screen SARS-CoV-2 variants
    Lekana-Douki, Sonia Etenna
    Pambou, Joa Braithe Mangombi
    N'dilimabaka, Nadine
    Minko, Ornella Zong
    Andeko, Julia Cyrielle
    Dikongo, Audrey Michel Ngonga
    Minko, Armand Edang
    Lekana-Douki, Jean-Bernard
    HELIYON, 2023, 9 (12)
  • [30] Complete Genome Sequence of a SARS-CoV-2 Strain Sampled in Morocco in May 2020, Obtained Using Sanger Sequencing
    Rfaki, Abderrazak
    Touil, Nadia
    Hemlali, Mouhssine
    Amine, Sanaa Alaoui
    Melloul, Marouane
    El Alaoui, Moulay Abdelaziz
    Elannaz, Hicham
    Lahlou, Amine Idriss
    Elouennass, Mostafa
    Ennibi, Khalid
    El Fahime, Elmostafa
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2021, 10 (20):
12345