Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector

被引:7
|
作者
Rossbach, Bella [1 ,2 ]
Hildebrand, Laura [1 ,2 ]
El-Ahmad, Linda [1 ,2 ,3 ]
Stachelscheid, Harald [1 ,2 ,3 ]
Reinke, Petra [1 ,2 ]
Kurtz, Andreas [1 ,2 ,4 ,5 ]
机构
[1] Charite, Augustenburger Pl 1, D-13353 Berlin, Germany
[2] Berlin Brandenburg Ctr Regenerat Therapies BCRT, Fohrer Str 15, D-13353 Berlin, Germany
[3] Berlin Inst Hlth, Stem Cell Core Facil, Berlin, Germany
[4] Seoul Natl Univ, Coll Vet Med, Seoul, South Korea
[5] Seoul Natl Univ, Res Inst Vet Sci, Seoul, South Korea
关键词
D O I
10.1016/j.scr.2015.12.018
中图分类号
Q813 [细胞工程];
学科分类号
摘要
We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 30 year old healthy female donor. The cells were reprogrammed using a non-integrating viral vector and have shown full differentiation potential. Together with the iPSC-line, the donor provided blood cells for the study of immunological effects of the iPSC line and its derivatives in autologous and allogeneic settings. The line is available and registered in the human pluripotent stem cell registry as BCRTi004-A. (C) 2016 The Authors. Published by Elsevier B.V.
引用
收藏
页码:314 / 317
页数:4
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