Highly Efficient Multi-Gene Knockout and Transgene Knock-in Using CRISPR-Cas12a in Induced Pluripotent Stem Cells for the Generation of Engineered Cell Immunotherapies

被引：0
|
作者：
Zuris, John A. [1 ]
Margulies, Carrie M. [1 ]
Viswanathan, Ramya [1 ]
Edelstein, Jasmine N. [1 ]
Pattali, Rithu [1 ]
Gareau, Kenneth W. [1 ]
Scott, Sean N. [1 ]
Lele, Swarali [1 ]
Wilson, Aaron C. [1 ]
Moon, Jungil I. [1 ]
Dilmac, Nejmi [1 ]
Friedland, Ari E. [1 ]
Morgan, Richard A. [1 ]
机构：
[1] Editas Med, Cambridge, MA USA
来源：
MOLECULAR THERAPY | 2020年 / 28卷 / 04期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
210
引用
收藏
页码：102 / 102
页数：1
相关论文
共 50 条
  • [41] Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression
    Schoger, Eric
    Zimmermann, Wolfram-Hubertus
    Cyganek, Lukas
    Zelarayan, Laura Cecilia
    STEM CELL RESEARCH, 2021, 55
  • [42] Gene editing and clonal isolation of human induced pluripotent stem cells using CRISPR/Cas9
    Yumlu, Saniye
    Stumm, Juergen
    Bashir, Sanum
    Dreyer, Anne-Kathrin
    Lisowski, Pawel
    Danner, Eric
    Kuehn, Ralf
    METHODS, 2017, 121 : 29 - 44
  • [43] Generation of NPHP1 knockout human pluripotent stem cells by a practical biallelic gene deletion strategy using CRISPR/Cas9 and ssODN
    Nakano, Yuta
    Susa, Koichiro
    Yanagi, Tomoki
    Hiraoka, Yuichi
    Suzuki, Takefumi
    Mori, Takayasu
    Ando, Fumiaki
    Mandai, Shintaro
    Fujiki, Tamami
    Rai, Tatemitsu
    Uchida, Shinichi
    Sohara, Eisei
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2022, 58 (02) : 85 - 95
  • [44] Generation of NPHP1 knockout human pluripotent stem cells by a practical biallelic gene deletion strategy using CRISPR/Cas9 and ssODN
    Yuta Nakano
    Koichiro Susa
    Tomoki Yanagi
    Yuichi Hiraoka
    Takefumi Suzuki
    Takayasu Mori
    Fumiaki Ando
    Shintaro Mandai
    Tamami Fujiki
    Tatemitsu Rai
    Shinichi Uchida
    Eisei Sohara
    In Vitro Cellular & Developmental Biology - Animal, 2022, 58 : 85 - 95
  • [45] Single-stranded DNA with internal base modifications mediates highly efficient knock-in in primary cells using CRISPR-Cas9
    Kanke, Karen L.
    Rayner, Rachael E.
    Bozik, Jack
    Abel, Eli
    Venugopalan, Aparna
    Suu, Ma
    Nouri, Reza
    Stack, Jacob T.
    Guo, Gongbo
    Vetter, Tatyana A.
    Cormet-Boyaka, Estelle
    Hester, Mark E.
    Vaidyanathan, Sriram
    NUCLEIC ACIDS RESEARCH, 2024, 52 (22) : 13561 - 13576
  • [46] Single-step generation of gene knockout-rescue system in pluripotent stem cells by promoter insertion with CRISPR/Cas9
    Matsunaga, Taichi
    Yamashita, Jun K.
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2014, 444 (02) : 158 - 163
  • [47] Generation of a FTO gene knockout human embryonic stem cell line using CRISPR/Cas9 editing
    Wei, Cong
    Luo, Qian
    Wang, Binsheng
    Long, Yan
    Zhang, Meng
    Shan, Wei
    Yu, Xiaohong
    Xu, Yulin
    Qian, Pengxu
    Huang, He
    STEM CELL RESEARCH, 2021, 53
  • [48] Robust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system
    Sho Yoshimatsu
    Junko Okahara
    Takefumi Sone
    Yuta Takeda
    Mari Nakamura
    Erika Sasaki
    Noriyuki Kishi
    Seiji Shiozawa
    Hideyuki Okano
    Scientific Reports, 9
  • [49] Generation and Characterization of CPVT1 Cardiomyocytes using Human Induced Pluripotent Stem Cells and CRISPR/Cas9 Gene Editing
    Yamaguchi, Naohiro
    Zhang, Xiao-Hua
    Wei, Hua
    Morad, Martin
    BIOPHYSICAL JOURNAL, 2018, 114 (03) : 116A - 116A
  • [50] Generation of a TMEM43 knockout human induced pluripotent stem cell line (HDZi003-A-1) using CRISPR/Cas9
    Ratnavadivel, Sandra
    Dammeier, Joline
    Gaertner, Anna
    de Toledo, Marcelo A. Szymanski
    Zenke, Martin
    Gummert, Jan
    Rasmussen, Torsten Bloch
    Klinke, Nora
    Juergens, Kai
    Meyer, Heiko
    Paululat, Achim
    Milting, Hendrik
    STEM CELL RESEARCH, 2024, 76
12345