Highly Efficient Multi-Gene Knockout and Transgene Knock-in Using CRISPR-Cas12a in Induced Pluripotent Stem Cells for the Generation of Engineered Cell Immunotherapies

被引：0
|
作者：
Zuris, John A. [1 ]
Margulies, Carrie M. [1 ]
Viswanathan, Ramya [1 ]
Edelstein, Jasmine N. [1 ]
Pattali, Rithu [1 ]
Gareau, Kenneth W. [1 ]
Scott, Sean N. [1 ]
Lele, Swarali [1 ]
Wilson, Aaron C. [1 ]
Moon, Jungil I. [1 ]
Dilmac, Nejmi [1 ]
Friedland, Ari E. [1 ]
Morgan, Richard A. [1 ]
机构：
[1] Editas Med, Cambridge, MA USA
来源：
MOLECULAR THERAPY | 2020年 / 28卷 / 04期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
210
引用
收藏
页码：102 / 102
页数：1
相关论文
共 50 条
  • [21] Protocol for CRISPR-Cas12a genome editing of protein tyrosine phosphatases in human pluripotent stem cells and functional (3-like cell generation
    Negueruela, Javier
    Vandenbempt, Valerie
    Talamantes, Stephanie
    Ribeiro-Costa, Francisco
    Nunes, Mariana
    Dias, Andre
    Bansal, Mayank
    Gurzov, Esteban N.
    STAR PROTOCOLS, 2024, 5 (03):
  • [22] Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9
    Han, Hyeong-jun
    Seo, Hyang-Hee
    Han, Hyo-Won
    Kim, Jung-Hyun
    STEM CELL RESEARCH, 2019, 40
  • [23] Generation of a NONO homozygous knockout human induced pluripotent stem cell line by CRISPR/Cas9 editing
    Yi, Tong
    Bai, Rui
    Sun, Hairui
    Lan, Feng
    Zhang, Hongjia
    He, Yihua
    STEM CELL RESEARCH, 2020, 47
  • [24] Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing
    Korneck, M.
    Leonhardt, A.
    Schoels, L.
    Hauser, S.
    STEM CELL RESEARCH, 2024, 77
  • [25] Author Correction: An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells
    Yifang Xie
    Daqi Wang
    Feng Lan
    Gang Wei
    Ting Ni
    Renjie Chai
    Dong Liu
    Shijun Hu
    Mingqing Li
    Dajin Li
    Hongyan Wang
    Yongming Wang
    Scientific Reports, 8
  • [26] Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system
    Kang, Ji -Young
    Mun, Dasom
    Chun, Yumin
    Park, Da -Seul
    Kim, Hyoeun
    Yun, Nuri
    Lee, Seung-Hyun
    Joung, Boyoung
    STEM CELL RESEARCH, 2022, 64
  • [27] A high throughput platform for CRISPR/Cas12a mediated manufacture of engineered induced pluripotent stem cell lines
    Blakemore, D.
    Pollack, S.
    Williams, J.
    Prangley, H.
    Forsberg, E.
    Ghezraoui, H.
    Jones, D.
    Toit, Y. D.
    Derizioti, P.
    Cawood, R.
    Brighty, D.
    HUMAN GENE THERAPY, 2022, 33 (23-24) : A151 - A151
  • [28] Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease
    Jung, Juwon
    Hwang, Sunsook
    Seol, Hyewon
    Kim, A-Hyeon
    Lee, Ha Myoung
    Sung, Jin Jea
    Jeong, Seung Min
    Choi, Young Min
    Jun, Jong Kwan
    Kim, Dong-wook
    Jang, Jiho
    STEM CELL RESEARCH, 2021, 53
  • [29] Generation of two LRRK2 homozygous knockout human induced pluripotent stem cell lines using CRISPR/Cas9
    Chen, Shuhan
    Luo, Zhiwei
    Ward, Carl
    Ibanez, David P.
    Liu, Hao
    Zhong, Xiaofen
    Sharma, Narendra K.
    Qin, Baoming
    Fan, Wenxia
    Wang, Dongye
    STEM CELL RESEARCH, 2020, 45
  • [30] Targeted Genome Editing of Human Induced Pluripotent Stem Cells Using CRISPR/CAS9 to Generate a Knock-in Type II Collagen Reporter for the Purification of Chondrogenic Cells
    Adkar, Shaunak S.
    Willard, Vincent P.
    Brunger, Jonathan M.
    Shiao, Kenneth T.
    Gersbach, Charles A.
    Guilak, Farshid
    MOLECULAR THERAPY, 2016, 24 : S128 - S128
12345