Quenched fluorescent peptide substrates - Tools for the discovery of novel biomarkers

被引：0
|
作者：
Lai, Zon W. [1 ]
Hanchapola, Iresha [1 ]
Smith, A. Ian [1 ]
机构：
[1] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia
来源：
CHIMICA OGGI-CHEMISTRY TODAY | 2008年 / 26卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The use of quenched fluorogenic labelled peptide substrates is growing and they have proved to be a valuable and effective tool for the rapid and sensitive screening for enzyme (particularly proteolytic) activity. Short synthetic peptides containing a quencher and the fluorophore provides high specificity and sensitivity to monitor enzyme cleavage and they thus have the potential to rapidly identify novel protease biomarkers for disease.
引用
收藏
页码：18 / 21
页数：4
相关论文
共 50 条
  • [1] Quenched Fluorescent Peptide Substrates as Tools for the Discovery of Novel Cardiovascular Disease Biomarkers
    Smith, A. Ian
    Warner, Fiona. J.
    Lew, Rebecca A.
    Yarski, Mike
    McGrath, Barry
    Burrell, Louise M.
    PEPTIDES FOR YOUTH, 2009, 611 : 419 - 422
  • [2] Quenched fluorescent peptide substrates as tools for the discovery of novel cardiovascular disease biomarkers
    Smith, A. I.
    Warner, F. J.
    Yarski, M.
    McGrath, B.
    Burrell, L. M.
    Lew, R. A.
    BIOPOLYMERS, 2007, 88 (04) : 525 - 525
  • [3] THE APPLICATION OF QUENCHED FLUORESCENT PEPTIDES FOR THE DISCOVERY OF BIOMARKERS FOR CARDIOVASCULAR DISEASE
    Smith, A. I.
    Kuruppu, S.
    Weng, Z. L.
    Burrell, L.
    CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2013, 40 : 7 - 7
  • [4] INTRAMOLECULARLY QUENCHED FLUORESCENT SUBSTRATES FOR ASPARTIC PROTEINASES
    FILIPPOVA, IY
    LYSOGORSKAYA, EN
    OKSENOIT, ES
    KOMAROV, YE
    STEPANOV, VM
    BIOORGANICHESKAYA KHIMIYA, 1986, 12 (09): : 1172 - 1180
  • [5] NEW INTRAMOLECULARLY QUENCHED FLUORESCENT SUBSTRATES FOR THE METALLOENDOPEPTIDASES
    FILIPPOVA, IY
    LYSOGORSKAYA, EN
    OKSENOIT, ES
    TROSHCHENKOVA, EP
    STEPANOV, VM
    BIOORGANICHESKAYA KHIMIYA, 1988, 14 (04): : 467 - 471
  • [6] An Internally Quenched Fluorescent Peptide Substrate for Protealysin
    Karaseva, Maria A.
    Chukhontseva, Ksenia N.
    Lemeskina, Irina S.
    Pridatchenko, Marina L.
    Kostrov, Sergey, V
    Demidyuk, Ilya, V
    SCIENTIFIC REPORTS, 2019, 9 (1)
  • [7] An Internally Quenched Fluorescent Peptide Substrate for Protealysin
    Maria A. Karaseva
    Ksenia N. Chukhontseva
    Irina S. Lemeskina
    Marina L. Pridatchenko
    Sergey V. Kostrov
    Ilya V. Demidyuk
    Scientific Reports, 9
  • [8] Proteolytic stability of β-peptide bonds probed using quenched fluorescent substrates incorporating a hemoglobin cleavage site
    Gopi, HN
    Ravindra, G
    Pal, PP
    Pattanaik, P
    Balaram, H
    Balaram, P
    FEBS LETTERS, 2003, 535 (1-3) : 175 - 178
  • [9] Discovery and validation of peptide biomarkers with novel mass spectrometry based workflows
    Krukf, Volker
    Rawer, Stephan
    Lenz, Christof
    JOURNAL OF PEPTIDE SCIENCE, 2008, 14 (08) : 113 - 113
  • [10] Internally quenched fluorescent peptide substrates disclose the subsite preferences of human caspases 1, 3, 6, 7 and 8
    Stennicke, HR
    Renatus, M
    Meldal, M
    Salvesen, GS
    BIOCHEMICAL JOURNAL, 2000, 350 : 563 - 568
12345