Production of 2-O-α-D-glucopyranosyl L-ascorbic acid using cyclodextrin glucanotransferase from Paenibacillus sp.

被引:27
|
作者
Jun, HK
Bae, KM
Kim, SK [1 ]
机构
[1] Pukyong Natl Univ, Dept Biotechnol & Bioengn, Pusan 608737, South Korea
[2] Pusan Natl Univ, Dept Biol Sci, Pusan 609735, South Korea
关键词
cyclodextrin glucanotransferase; 2-O-alpha-D-glucopyranosyl L-ascorbic acid; Paenibacillus sp; transglycosylation;
D O I
10.1023/A:1012413301061
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Transglycosylation to produce a 2-O-alpha -d-glucopyranosyl L-ascorbic acid (AA-2G) was studied using cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. A series of maltooligosaccharides substituted 2-O-derivatives of L-ascorbic acid (AA) were analyzed by HPLC. The maltooligosaccharides were hydrolyzed by glucoamylase to give AA-2G. CGTase also produced AA-2G using dextrin as a glycosyl donor and AA as an acceptor. CGTase utilized alpha-, beta-, and gamma -CDs, amylose, soluble starch and corn starch as glycosyl donors but not glucose.
引用
收藏
页码:1793 / 1797
页数:5
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