Production of 2-O-α-D-glucopyranosyl L-ascorbic acid using cyclodextrin glucanotransferase from Paenibacillus sp.

被引：27
|
作者：
Jun, HK
Bae, KM
Kim, SK [1 ]
机构：
[1] Pukyong Natl Univ, Dept Biotechnol & Bioengn, Pusan 608737, South Korea
[2] Pusan Natl Univ, Dept Biol Sci, Pusan 609735, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 21期
关键词：
cyclodextrin glucanotransferase; 2-O-alpha-D-glucopyranosyl L-ascorbic acid; Paenibacillus sp; transglycosylation;
D O I：
10.1023/A:1012413301061
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Transglycosylation to produce a 2-O-alpha -d-glucopyranosyl L-ascorbic acid (AA-2G) was studied using cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. A series of maltooligosaccharides substituted 2-O-derivatives of L-ascorbic acid (AA) were analyzed by HPLC. The maltooligosaccharides were hydrolyzed by glucoamylase to give AA-2G. CGTase also produced AA-2G using dextrin as a glycosyl donor and AA as an acceptor. CGTase utilized alpha-, beta-, and gamma -CDs, amylose, soluble starch and corn starch as glycosyl donors but not glucose.
引用
收藏
页码：1793 / 1797
页数：5
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