Glassy carbon electrode modified with 3D graphene-carbon nanotube network for sensitive electrochemical determination of methotrexate

In the present study, a 3D porous graphene-carbon nanotube (G-CNT) network is successfully constructed on the surface of glassy carbon electrode (GCE) by electrochemical co-deposition from a concentrated graphene dispersion. The large accessible surface area provided by the interpenetrated graphene backbone in one hand and the enhanced electrical conductivity of the 3D network by incorporating CNTs on the other hand, dramatically improved the electrochemical performance of GCE in determination of Methotrexate (MTX) as an important electroactive drug compound. Under the optimum conditions, the electrode modification led to a significant increase in the anodic peak current (similar to 25 times) along with a considerable shift in the peak potential (similar to 111 mV). Voltammetric investigations revealed that the proposed method can determine MTX in a wide dynamic linear range with a low detection limit of 70 nM. Moreover, good sensitivity and high accuracy of the prepared modified electrode in voltammetric detections of MTX, which was further confirmed by UV-vis spectroscopy and HPLC methods, make it very suitable for accurate determinations of MTX in pharmaceutical formulations (commercial tablets) and clinical preparations (blood serum) with excellent recoveries. (C) 2016 Elsevier B.V. All rights reserved.