Hep3B human hepatoma cells support replication of the wild-type and a 5′-end deletion mutant GB virus B replicon

被引:6
|
作者
De Tomassi, A [1 ]
Pizzuti, M [1 ]
Traboni, C [1 ]
机构
[1] Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Rome, Italy
关键词
HEPATITIS-C VIRUS; 5' NONTRANSLATED REGION; RNA REPLICATION; TRANSLATION; SEQUENCES; GENOME; AGENT; CLONE;
D O I
10.1128/JVI.77.22.11875-11881.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hepatitis C virus (HCV) and GB virus B (GBV-B) replicons have been reported to replicate only in Hub7 cells. Here we demonstrate that subpopulations of another human hepatoma cell line, Hep3B, are permissive for the GBV-B replicon, showing different levels of enhancement of replication from those of the unselected parental cell population. Adaptive mutations are not required for replication of the GBV-B replicon in these cells, as already demonstrated for Huh7 cells. Nonetheless, we identified a mutant replicon in one of the selected cell lines, which, although lacking the 5' end proximal stem-loop, is able to replicate in Hep3B cells as well as in Huh7 cells. This mutant indeed shows a higher replication efficiency than does wild-type replicon, especially in the Hep3B cell clone from which it was originally recovered. This indicates that the stem-loop la is not necessary for replication of the GBV-B replicon in human cells, unlike what occurs with HCV, and that its absence can even provide a selective advantage.
引用
收藏
页码:11875 / 11881
页数:7
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