Hep3B human hepatoma cells support replication of the wild-type and a 5′-end deletion mutant GB virus B replicon

被引:6
|
作者
De Tomassi, A [1 ]
Pizzuti, M [1 ]
Traboni, C [1 ]
机构
[1] Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Rome, Italy
关键词
HEPATITIS-C VIRUS; 5' NONTRANSLATED REGION; RNA REPLICATION; TRANSLATION; SEQUENCES; GENOME; AGENT; CLONE;
D O I
10.1128/JVI.77.22.11875-11881.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hepatitis C virus (HCV) and GB virus B (GBV-B) replicons have been reported to replicate only in Hub7 cells. Here we demonstrate that subpopulations of another human hepatoma cell line, Hep3B, are permissive for the GBV-B replicon, showing different levels of enhancement of replication from those of the unselected parental cell population. Adaptive mutations are not required for replication of the GBV-B replicon in these cells, as already demonstrated for Huh7 cells. Nonetheless, we identified a mutant replicon in one of the selected cell lines, which, although lacking the 5' end proximal stem-loop, is able to replicate in Hep3B cells as well as in Huh7 cells. This mutant indeed shows a higher replication efficiency than does wild-type replicon, especially in the Hep3B cell clone from which it was originally recovered. This indicates that the stem-loop la is not necessary for replication of the GBV-B replicon in human cells, unlike what occurs with HCV, and that its absence can even provide a selective advantage.
引用
收藏
页码:11875 / 11881
页数:7
相关论文
共 50 条
  • [21] Mutant p53 can provoke apoptosis in p53-deficient Hep3B cells with delayed kinetics relative to wild-type p53
    Stähler, F
    Roemer, K
    ONCOGENE, 1998, 17 (26) : 3507 - 3512
  • [22] Overexpression of the mouse Fas gene in human Hep3B hepatoma cells overcomes their resistance to Fas-mediated apoptosis
    Lamboley, C
    Bringuier, AF
    Camus, E
    Lardeux, B
    Groyer, A
    Feldmann, G
    JOURNAL OF HEPATOLOGY, 2002, 36 (03) : 385 - 394
  • [23] The role of hypoxia response element in TGFβ-induced carbonic anhydrase IX expression in Hep3B human hepatoma cells
    Yildirim, Hatice
    Karaman, Merve
    Kockar, Feray
    ARCHIVES OF BIOLOGICAL SCIENCES, 2017, 69 (04) : 593 - 601
  • [24] Retinoic acid-induced apoptosis is prevented by serum albumin and enhanced by Lipiodol in human hepatoma Hep3B cells
    Hsu, SL
    Wu, WS
    Tyan, YS
    Chou, CK
    CANCER LETTERS, 1998, 129 (02) : 205 - 214
  • [25] Genotoxic effects of dietary and lifestyle related carcinogens in human derived hepatoma (HepG2, Hep3B) cells
    Majer, BJ
    Mersch-Sundermann, V
    Darroudi, F
    Laky, B
    de Wit, K
    Knasmüller, S
    MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2004, 551 (1-2) : 153 - 166
  • [26] Cytotoxic and apoptotic activity of Cheilanthes farinosa (Forsk.) Kaulf. against human hepatoma, Hep3B cells
    Radhika, N. Krishna
    Sreejith, P. S.
    Asha, V. V.
    JOURNAL OF ETHNOPHARMACOLOGY, 2010, 128 (01) : 166 - 171
  • [27] Inhibition of hepatitis B virus (HBV) replication by pyrimidines bearing an acyclic moiety: Effect on wild-type and mutant HBV
    Semaine, W
    Johar, M
    Tyrrell, DLJ
    Kumar, R
    Agrawal, B
    JOURNAL OF MEDICINAL CHEMISTRY, 2006, 49 (06) : 2049 - 2054
  • [28] Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase
    You, Bang-Jau
    Hour, Mann-Jen
    Chen, Li-Yun
    Luo, Shu-Ching
    Hsu, Po-Hsiang
    Lee, Hong-Zin
    SCIENTIFIC REPORTS, 2019, 9 (1)
  • [29] Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase
    Bang-Jau You
    Mann-Jen Hour
    Li-Yun Chen
    Shu-Ching Luo
    Po-Hsiang Hsu
    Hong-Zin Lee
    Scientific Reports, 9
  • [30] Etoposide induces a mixed type of programmed cell death and overcomes the resistance conferred by Bcl-2 in Hep3B hepatoma cells
    Yoo, Seung Hee
    Yoon, Young Geol
    Lee, Jee Suk
    Song, Yeon Suk
    Oh, Joon Seok
    Park, Bong Soo
    Kwon, Taeg Kyu
    Park, Cheol
    Choi, Yung Hyun
    Yoo, Young Hyun
    INTERNATIONAL JOURNAL OF ONCOLOGY, 2012, 41 (04) : 1443 - 1454