P2X7 receptor stimulation of membrane internalization in a thyrocyte cell line

被引:14
|
作者
Kochukov, MY [1 ]
Ritchie, AK [1 ]
机构
[1] Univ Texas, Med Branch, Dept Physiol & Biophys, Galveston, TX 77555 USA
来源
JOURNAL OF MEMBRANE BIOLOGY | 2005年 / 204卷 / 01期
关键词
P2X7; receptor; ATP; endocytosis; thyroid; patch clamp;
D O I
10.1007/s00232-005-0742-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using fluorescent membrane markers, we have previously shown that extracellular ATP stimulates both exocytosis and membrane internalization in the Fisher rat thyroid cell line FRTL. In this study, we examine the actions of ATP using whole-cell recording conditions that favor stimulation of membrane internalization. ATP stimulation of the P2X(7) receptor activated a reversible, Ca2+ -permeable, cation conductance that slowly increased in size without changes in ion selectivity. ATP also induced a delayed irreversible decrease in cell capacitance (C.) that was equivalent to an 8% decrease in membrane surface area. Addition of guanosine 5'-O-2-thiodiphosphate to the pipette solution inhibited the ATP-induced decrease in C. without affecting channel activation. The effects of ATP on membrane conductance were mimicked by 2',3'-O-(4-benzoylbenzoyl)-ATP, but not by UTP, adenosine, or 2-methylthio-ATP, and were inhibited by pyridoxal phosphate-6-azophenyl-2'4'-disulfoiilc acid, adenosine 5'-triphosphate-2'3'-dialdehyde, and Cu2+. The capacitance decrease persisted in Na+-, Ca2+- and Cl--free external saline or with Ca2+-free pipette solution. It is concluded that ATP activation of the inotropic P2X(7) receptor stimulates membrane internalization by a mechanism that involves intracellular GTP, but does not require internal Ca2+ or influx of Na+ or Ca2+ through the receptor-gated channel.
引用
收藏
页码:11 / 21
页数:11
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