P2X7 receptor stimulation of membrane internalization in a thyrocyte cell line

被引:14
|
作者
Kochukov, MY [1 ]
Ritchie, AK [1 ]
机构
[1] Univ Texas, Med Branch, Dept Physiol & Biophys, Galveston, TX 77555 USA
来源
JOURNAL OF MEMBRANE BIOLOGY | 2005年 / 204卷 / 01期
关键词
P2X7; receptor; ATP; endocytosis; thyroid; patch clamp;
D O I
10.1007/s00232-005-0742-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using fluorescent membrane markers, we have previously shown that extracellular ATP stimulates both exocytosis and membrane internalization in the Fisher rat thyroid cell line FRTL. In this study, we examine the actions of ATP using whole-cell recording conditions that favor stimulation of membrane internalization. ATP stimulation of the P2X(7) receptor activated a reversible, Ca2+ -permeable, cation conductance that slowly increased in size without changes in ion selectivity. ATP also induced a delayed irreversible decrease in cell capacitance (C.) that was equivalent to an 8% decrease in membrane surface area. Addition of guanosine 5'-O-2-thiodiphosphate to the pipette solution inhibited the ATP-induced decrease in C. without affecting channel activation. The effects of ATP on membrane conductance were mimicked by 2',3'-O-(4-benzoylbenzoyl)-ATP, but not by UTP, adenosine, or 2-methylthio-ATP, and were inhibited by pyridoxal phosphate-6-azophenyl-2'4'-disulfoiilc acid, adenosine 5'-triphosphate-2'3'-dialdehyde, and Cu2+. The capacitance decrease persisted in Na+-, Ca2+- and Cl--free external saline or with Ca2+-free pipette solution. It is concluded that ATP activation of the inotropic P2X(7) receptor stimulates membrane internalization by a mechanism that involves intracellular GTP, but does not require internal Ca2+ or influx of Na+ or Ca2+ through the receptor-gated channel.
引用
收藏
页码:11 / 21
页数:11
相关论文
共 50 条
  • [21] P2X7 receptor: Death or life?
    Adinolfi E.
    Pizzirani C.
    Idzko M.
    Panther E.
    Norgauer J.
    Di Virgilio F.
    Ferrari D.
    Purinergic Signalling, 2005, 1 (3) : 219 - 227
  • [22] P2X7 receptor in inflammation and pain
    Hu, Shi-qi
    Hu, Jia-ling
    Zou, Fei-long
    Liu, Ji-peng
    Luo, Hong-liang
    Hu, Dong-xia
    Wu, Li-dong
    Zhang, Wen-jun
    BRAIN RESEARCH BULLETIN, 2022, 187 : 199 - 209
  • [23] The P2X7 Receptor in Tumor Immunity
    Grassi, Fabio
    Conti, Benedetta De Ponte
    FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2021, 9
  • [24] The P2X7 Receptor in Infection and Inflammation
    Di Virgilio, Francesco
    Dal Ben, Diego
    Sarti, Alba Clara
    Giuliani, Anna Lisa
    Falzoni, Simonetta
    IMMUNITY, 2017, 47 (01) : 15 - 31
  • [25] P2X7 receptor - the communicator in the cornea
    Trinkaus-Randall, Vickery E.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2019, 60 (09)
  • [26] P2X7 Receptor as a Therapeutic Target
    De Marchi, Elena
    Orioli, Elisa
    Dal Ben, Diego
    Adinolfi, Elena
    ION CHANNELS AS THERAPEUTIC TARGETS, PT B, 2016, 104 : 39 - 79
  • [27] P2X7 receptor and polykarion formation
    Falzoni, S
    Chiozzi, P
    Ferrari, D
    Buell, G
    Di Virgilio, F
    MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (09) : 3169 - 3176
  • [28] Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line
    Takenouchi, Takato
    Iwamaru, Yoshifumi
    Imamura, Morikazu
    Kato, Nobuko
    Sugama, Shuei
    Fujita, Masayo
    Hashimoto, Makoto
    Sato, Mitsuru
    Okada, Hiroyuki
    Yokoyama, Takashi
    Mohri, Shirou
    Kitani, Hiroshi
    FEBS LETTERS, 2007, 581 (16) : 3019 - 3026
  • [29] Novel P2X7 receptor antagonists
    Alcaraz, L
    Baxter, A
    Bent, J
    Bowers, K
    Braddock, M
    Cladingboel, D
    Donald, D
    Fagura, M
    Furber, M
    Laurent, C
    Lawson, M
    Mortimore, M
    McCormick, M
    Roberts, N
    Robertson, M
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2003, 13 (22) : 4043 - 4046
  • [30] P2X7 Receptor in Hematological Malignancies
    De Marchi, Elena
    Pegoraro, Anna
    Adinolfi, Elena
    FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2021, 9