Partial Epithelial-Mesenchymal Transition Was Observed Under p63 Expression in Acquired Middle Ear Cholesteatoma and Congenital Cholesteatoma

被引:9
|
作者
Takahashi, Masahiro [1 ]
Yamamoto-Fukuda, Tomomi [1 ]
Akiyama, Naotaro [2 ]
Motegi, Masaomi [1 ]
Yamamoto, Kazuhisa [1 ]
Tanaka, Yasuhiro [3 ]
Yamamoto, Yutaka [1 ]
Kojima, Hiromi [1 ]
机构
[1] Jikei Univ, Dept Otorhinolaryngol, Sch Med, Tokyo, Japan
[2] Toho Univ, Dept Otorhinolaryngol, Sch Med, Tokyo, Japan
[3] Dokkyo Med Univ, Dept Otorhinolaryngol, Saitama Med Ctr, Saitama, Japan
关键词
Acquired middle ear cholesteatoma; Congenital cholesteatoma; E-cadherin; Immunohistochemistry; p63; Partial EMT; CELLS; ORGANOGENESIS; METASTASIS; MIGRATION; CLAUDINS; RECEPTOR; EMT;
D O I
10.1097/MAO.0000000000002328
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Introduction: Partial epithelial-mesenchymal transition (p-EMT) is a process by which epithelial cells partially lose their intercellular adhesion and change to obtain migration ability. The transcription factor p63 regulates the expression of cadherin family and induces epithelial cell proliferation. In this study, we hypothesized that p-EMT under p63 expression may be a key factor in epithelial cell growth in middle ear cholesteatoma. Methods: Specimens were surgically excised from patients with congenital cholesteatoma (CC) (n = 48), acquired middle ear cholesteatoma (AC) (n = 120), and normal skin tissue (n = 34). We analyzed immunohistochemically for the EMT marker (N-cadherin), adherence junction marker (E-cadherin), and tight junction marker (claudin-1, claudin-4, occludin). We also examined the labeling index (LI) of p63 and Proliferating cell nuclear antigen (PCNA) (late S phase marker), and Snail expression as a mobility marker. Results: The expression of p63 (CC 51.0 +/- 7.4%, AC 50.0 +/- 5.9%) was significantly higher in the thickened epithelium of CC and AC compared with normal skin tissue (p < 0.0001). The loss of E-cadherin was observed (CC 50.0%, AC 55.8%) but the expression patterns in the tight junction were almost normal. N-cadherin was partially detected in the basal and upper layer of epithelium in CC and AC. In contrast to that of normal skin tissue, the LI of PCNA was significantly higher in AC (p < 0.0001). The positive rate of Snail was significantly higher in CC (p < 0.0001). Conclusion: This study indicates that p-EMT via the p63 signaling pathway might plays an essential role in epithelial growth in AC and CC formation, although tight junction formation and terminal differentiation were not affected in those processes.
引用
收藏
页码:E803 / E811
页数:9
相关论文
共 50 条
  • [21] The p63 Protein Isoform ΔNp63α Inhibits Epithelial-Mesenchymal Transition in Human Bladder Cancer Cells ROLE OF MIR-205
    Tran, Mai N.
    Choi, Woonyoung
    Wszolek, Matthew F.
    Navai, Neema
    Lee, I-Ling C.
    Nitti, Giovanni
    Wen, Sijin
    Flores, Elsa R.
    Siefker-Radtke, Arlene
    Czerniak, Bogdan
    Dinney, Colin
    Barton, Michelle
    McConkey, David J.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (05) : 3275 - 3288
  • [22] p63 inhibits epithelial - mesenchymal transition by promoting the expression of miR205 in human bladder cancer cells
    Tran, Mai N.
    Choi, Woonyoung
    Nayai, Neema
    Wszolek, Matthew F.
    Lee, I-Iing C.
    Siefker-Radtke, Arlene O.
    McConkey, David J.
    CANCER RESEARCH, 2012, 72
  • [23] Immunohistochemical localization of d-b-aspartic acid in congenital and acquired middle ear cholesteatoma (vol 7, pg 1155, 2022)
    Kitaya, S.
    Ikeda, R.
    Suzuki, J.
    LARYNGOSCOPE INVESTIGATIVE OTOLARYNGOLOGY, 2023, 8 (03): : 792 - 792
  • [24] TFIIB-related factor 2 regulates glucose-regulated protein 78 expression in acquired middle ear cholesteatoma
    Sui, RongCui
    Liu, ChengCheng
    Wang, Na
    Fan, XinTai
    Han, ShuHui
    Zhang, Jie
    Hou, LingXiao
    Zhang, XianZhao
    Xu, AnTing
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021, 540 : 95 - 100
  • [25] The ability of the transcription factor p63 to induce selected gene expression modules associated with mesenchymal to epithelial transition of prostate cells
    Olsen, Jan Roger
    Ke, Xi-Song
    Rostad, Kari
    Hellem, Margrete R.
    Qu, Yi
    Lin, Biaoyang
    Lorens, James B.
    Micklem, David R.
    Haugen, Hallvard
    Gravdal, Karsten
    Halvorsen, Ole Johan
    Akslen, Lars A.
    Rotter, Varda
    Oyan, Anne Margrete
    Kalland, Karl H.
    CANCER RESEARCH, 2012, 72
  • [26] Expression and Correlation Research of MicroRNA-10a-5p and PIK3CA in Middle Ear Cholesteatoma
    Yang, Jing
    Yan, Wei
    Tang, Susu
    Huang, Zhikun
    Ye, Mingyuan
    Lu, Zheng
    Liu, Qianxu
    JOURNAL OF INTERNATIONAL ADVANCED OTOLOGY, 2023, 19 (03): : 212 - +
  • [27] Possible involvement of keratinocyte growth factor and its receptor in enhanced epithelial-cell proliferation and acquired recurrence of middle-ear cholesteatoma
    Yamamoto-Fukuda, T
    Aoki, D
    Hishikawa, Y
    Kobayashi, T
    Takahashi, H
    Koji, T
    LABORATORY INVESTIGATION, 2003, 83 (01) : 123 - 136
  • [28] ANTIGEN EXPRESSION OF EPITHELIAL MARKERS, COLLAGEN-IV AND COLLAGEN-KI67 IN MIDDLE-EAR CHOLESTEATOMA - AN IMMUNOHISTOCHEMICAL STUDY
    ERGUN, S
    ZHENG, X
    CARLSOO, B
    ACTA OTO-LARYNGOLOGICA, 1994, 114 (03) : 295 - 302
  • [29] TP63 (p63) knockout induces epithelial mesenchymal transition through DNA methylation control in squamous cell carcinoma
    Katoh, Iyoko
    Hata, Ryuichiro
    Kurata, Shunichi
    CANCER SCIENCE, 2023, 114 : 1326 - 1326
  • [30] Epithelial-mesenchymal transition markers and P53 expression in urothelial bladder tumors
    Bravou, V.
    Aroukatos, P.
    Nakas, D.
    Athanasopoulou, A.
    Kotsikogianni, I.
    Verras, D.
    Damaskou, V.
    Vandoros, G.
    Repanti, M.
    HISTOPATHOLOGY, 2008, 53 : 269 - 269