Partial Epithelial-Mesenchymal Transition Was Observed Under p63 Expression in Acquired Middle Ear Cholesteatoma and Congenital Cholesteatoma

被引:9
|
作者
Takahashi, Masahiro [1 ]
Yamamoto-Fukuda, Tomomi [1 ]
Akiyama, Naotaro [2 ]
Motegi, Masaomi [1 ]
Yamamoto, Kazuhisa [1 ]
Tanaka, Yasuhiro [3 ]
Yamamoto, Yutaka [1 ]
Kojima, Hiromi [1 ]
机构
[1] Jikei Univ, Dept Otorhinolaryngol, Sch Med, Tokyo, Japan
[2] Toho Univ, Dept Otorhinolaryngol, Sch Med, Tokyo, Japan
[3] Dokkyo Med Univ, Dept Otorhinolaryngol, Saitama Med Ctr, Saitama, Japan
关键词
Acquired middle ear cholesteatoma; Congenital cholesteatoma; E-cadherin; Immunohistochemistry; p63; Partial EMT; CELLS; ORGANOGENESIS; METASTASIS; MIGRATION; CLAUDINS; RECEPTOR; EMT;
D O I
10.1097/MAO.0000000000002328
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Introduction: Partial epithelial-mesenchymal transition (p-EMT) is a process by which epithelial cells partially lose their intercellular adhesion and change to obtain migration ability. The transcription factor p63 regulates the expression of cadherin family and induces epithelial cell proliferation. In this study, we hypothesized that p-EMT under p63 expression may be a key factor in epithelial cell growth in middle ear cholesteatoma. Methods: Specimens were surgically excised from patients with congenital cholesteatoma (CC) (n = 48), acquired middle ear cholesteatoma (AC) (n = 120), and normal skin tissue (n = 34). We analyzed immunohistochemically for the EMT marker (N-cadherin), adherence junction marker (E-cadherin), and tight junction marker (claudin-1, claudin-4, occludin). We also examined the labeling index (LI) of p63 and Proliferating cell nuclear antigen (PCNA) (late S phase marker), and Snail expression as a mobility marker. Results: The expression of p63 (CC 51.0 +/- 7.4%, AC 50.0 +/- 5.9%) was significantly higher in the thickened epithelium of CC and AC compared with normal skin tissue (p < 0.0001). The loss of E-cadherin was observed (CC 50.0%, AC 55.8%) but the expression patterns in the tight junction were almost normal. N-cadherin was partially detected in the basal and upper layer of epithelium in CC and AC. In contrast to that of normal skin tissue, the LI of PCNA was significantly higher in AC (p < 0.0001). The positive rate of Snail was significantly higher in CC (p < 0.0001). Conclusion: This study indicates that p-EMT via the p63 signaling pathway might plays an essential role in epithelial growth in AC and CC formation, although tight junction formation and terminal differentiation were not affected in those processes.
引用
收藏
页码:E803 / E811
页数:9
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