DNA topological change in the hyperthermophilic archaeon Pyrococcus abyssi exposed to low temperature

被引:10
|
作者
Marguet, E [1 ]
Zivanovic, Y [1 ]
Forterre, P [1 ]
机构
[1] UNIV PARIS 11,CNRS,INST GENET & MICROBIOL,URA 1354,F-91405 ORSAY,FRANCE
关键词
hyperthermophile; archaebacteria; DNA topology; DNA topoisomerases; plasmid supercoiling; DNA supercoiling; cold-shock; reverse gyrase;
D O I
10.1111/j.1574-6968.1996.tb08403.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Topology of the plasmid pGT5 from the hyperthermophilic archaeon Pyrococcus abyssi has been studied as a function of growth phase and temperature. Plasmids isolated from cells in mid-exponential or stationary phases had similar linking number (Lk), whilst plasmids isolated from cells grown al 95 degrees C had a higher Lk than those isolated from cells grown at 80 degrees C. A significant reduction of Lk was observed when cells cultivated at 95 degrees C were slowly cooled at room temperature.
引用
收藏
页码:31 / 36
页数:6
相关论文
共 50 条
  • [31] Recombinant production and characterization of a metal ion-independent Lysophospholipase from a hyperthermophilic archaeon Pyrococcus abyssi DSM25543
    Nazir, Arshia
    Shad, Mohsin
    Rashid, Naeem
    Azim, Naseema
    Sajjad, Muhammad
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 259
  • [32] A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization
    Uemori, T
    Sato, Y
    Kato, I
    Doi, H
    Ishino, Y
    GENES TO CELLS, 1997, 2 (08) : 499 - 512
  • [33] Domain topology of the DNA polymerase D complex from a hyperthermophilic Archaeon Pyrococcus horikoshii
    Tang, XF
    Shen, YL
    Matsui, E
    Matsui, I
    BIOCHEMISTRY, 2004, 43 (37) : 11818 - 11827
  • [34] A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus
    Imamura, M
    Uemori, T
    Kato, I
    Ishino, Y
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1995, 18 (12) : 1647 - 1652
  • [35] MINIMAL AMINO-ACID-REQUIREMENTS OF THE HYPERTHERMOPHILIC ARCHAEON PYROCOCCUS-ABYSSI, ISOLATED FROM DEEP-SEA HYDROTHERMAL VENTS
    WATRIN, L
    MARTINJEZEQUEL, V
    PRIEUR, D
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (03) : 1138 - 1140
  • [36] Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1
    Hashimoto, H
    Nishioka, M
    Fujiwara, S
    Takagi, M
    Imanaka, T
    Inoue, T
    Kai, Y
    JOURNAL OF MOLECULAR BIOLOGY, 2001, 306 (03) : 469 - 477
  • [38] Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization
    Branco, Roberta V.
    Gutarra, Melissa L. E.
    Guisan, Jose M.
    Freire, Denise M. G.
    Almeida, Rodrigo V.
    Palomo, Jose M.
    BIOMED RESEARCH INTERNATIONAL, 2015, 2015
  • [39] Crystallization and preliminary X-ray analysis of a DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii
    Ito, N
    Nureki, O
    Shirouzu, M
    Yokoyama, S
    Hanaoka, F
    JOURNAL OF BIOCHEMISTRY, 2001, 130 (06): : 727 - 730
  • [40] EXTREME RESISTANCE TO THERMALLY-INDUCED DNA BACKBONE BREAKS IN THE HYPERTHERMOPHILIC ARCHAEON PYROCOCCUS-FURIOSUS
    PEAK, MJ
    ROBB, FT
    PEAK, JG
    JOURNAL OF BACTERIOLOGY, 1995, 177 (21) : 6316 - 6318