DNA topological change in the hyperthermophilic archaeon Pyrococcus abyssi exposed to low temperature

被引：10
|
作者：
Marguet, E [1 ]
Zivanovic, Y [1 ]
Forterre, P [1 ]
机构：
[1] UNIV PARIS 11,CNRS,INST GENET & MICROBIOL,URA 1354,F-91405 ORSAY,FRANCE
来源：
FEMS MICROBIOLOGY LETTERS | 1996年 / 142卷 / 01期
关键词：
hyperthermophile; archaebacteria; DNA topology; DNA topoisomerases; plasmid supercoiling; DNA supercoiling; cold-shock; reverse gyrase;
D O I：
10.1111/j.1574-6968.1996.tb08403.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Topology of the plasmid pGT5 from the hyperthermophilic archaeon Pyrococcus abyssi has been studied as a function of growth phase and temperature. Plasmids isolated from cells in mid-exponential or stationary phases had similar linking number (Lk), whilst plasmids isolated from cells grown al 95 degrees C had a higher Lk than those isolated from cells grown at 80 degrees C. A significant reduction of Lk was observed when cells cultivated at 95 degrees C were slowly cooled at room temperature.
引用
收藏
页码：31 / 36
页数：6
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