Construction and validation of a neutrally-marked strain of Pseudomonas fluorescens SBW25

被引：45
|
作者：
Zhang, Xue-Xian
Rainey, Paul B.
机构：
[1] Massey Univ, NZ Inst Adv Study, Auckland, New Zealand
[2] Massey Univ, Inst Mol Biosci, Auckland, New Zealand
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 71卷 / 01期
关键词：
lacZ; relative fitness; Pseudomonas fluorescens SBW25; selection rate constant;
D O I：
10.1016/j.mimet.2007.07.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Neutrally marked bacterial strains are useful in many experimental evolution and molecular ecology studies to assess the relative fitness of a given strain. Here we describe the construction and validation of a neutral marker for the model organism Pseudomonas fluorescens SBW25. The marked strain, called SBW25-lacZ, was created by integrating a promoterless 'lacZ into the defective prophage locus of the SBW25 chromosome. Fitness assays conducted in various laboratory media and in planta revealed that the fitness levels of SBW25-lacZ were comparable with the wildtype ancestor. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：78 / 81
页数：4
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