Large-scale imaging of cortical network activity with calcium indicators

被引：99
|
作者：
Ikegaya, Y [1 ]
Le Bon-Jego, M [1 ]
Yuste, R [1 ]
机构：
[1] Columbia Univ, Dept Biol Sci, Fairchild Ctr 1002, New York, NY 10027 USA
来源：
NEUROSCIENCE RESEARCH | 2005年 / 52卷 / 02期
关键词：
slice preparation; cortex; fluo-4; Oregon green; spike; microcircuitry; action potential; circuit; spinning disk; confocal microscope;
D O I：
10.1016/j.neures.2005.02.004
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
引用
收藏
页码：132 / 138
页数：7
相关论文
共 50 条
  • [41] ON LARGE-SCALE NONLINEAR NETWORK OPTIMIZATION
    TOINT, PL
    TUYTTENS, D
    MATHEMATICAL PROGRAMMING, 1990, 48 (01) : 125 - 159
  • [42] LARGE-SCALE NETWORK TOPOLOGICAL OPTIMIZATION
    BOORSTYN, RR
    FRANK, H
    IEEE TRANSACTIONS ON COMMUNICATIONS, 1977, 25 (01) : 29 - 47
  • [43] A network architecture for large-scale science
    Gorinsky, Sergey
    Jechlitschek, Christoph
    Rao, Nageswara S. V.
    2007 HIGH-SPEED NETWORKS WORKSHOP, 2007, : 1 - +
  • [44] A LARGE-SCALE NETWORK STORAGE FACILITY
    DAKIN, RJ
    LEDERER, BR
    PARKER, KR
    SOFTWARE-PRACTICE & EXPERIENCE, 1985, 15 (09): : 889 - 899
  • [45] The Case for a Large-scale Occultation Network
    Rice, Malena
    Laughlin, Gregory
    ASTRONOMICAL JOURNAL, 2019, 158 (01):
  • [46] Large-Scale Signaling Network Reconstruction
    Hashemikhabir, Seyedsasan
    Ayaz, Eyup Serdar
    Kavurucu, Yusuf
    Can, Tolga
    Kahveci, Tamer
    IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS, 2012, 9 (06) : 1696 - 1708
  • [47] Indicators of reactors in large-scale nuclear power in Russia
    E. O. Adamov
    I. Kh. Ganev
    A. V. Lopatkin
    V. G. Muratov
    V. V. Orlov
    Atomic Energy, 1998, 85 : 769 - 775
  • [48] Indicators of reactors in large-scale nuclear power in Russia
    Adamov, EO
    Ganev, IK
    Lopatkin, AV
    Muratov, VG
    Orlov, VV
    ATOMIC ENERGY, 1998, 85 (05) : 769 - 775
  • [49] Automatic Cell Segmentation by Adaptive Thresholding (ACSAT) for Large-Scale Calcium Imaging Datasets
    Shen, Simon P.
    Tseng, Hua-an
    Hansen, Kyle R.
    Wu, Ruofan
    Gritton, Howard J.
    Si, Jennie
    Han, Xue
    ENEURO, 2018, 5 (05)
  • [50] Large-scale calcium waves in the developing brain
    Konnerth, A
    Garaschuk, O
    EUROPEAN JOURNAL OF NEUROSCIENCE, 2000, 12 : 516 - 516
12345