Large-scale imaging of cortical network activity with calcium indicators

被引：99
|
作者：
Ikegaya, Y [1 ]
Le Bon-Jego, M [1 ]
Yuste, R [1 ]
机构：
[1] Columbia Univ, Dept Biol Sci, Fairchild Ctr 1002, New York, NY 10027 USA
来源：
NEUROSCIENCE RESEARCH | 2005年 / 52卷 / 02期
关键词：
slice preparation; cortex; fluo-4; Oregon green; spike; microcircuitry; action potential; circuit; spinning disk; confocal microscope;
D O I：
10.1016/j.neures.2005.02.004
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
引用
收藏
页码：132 / 138
页数：7
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