A novel immunosensor for Enterobacter sakazakii based on multiwalled carbon nanotube/ionic liquid/thionine modified electrode

被引：23
|
作者：
Zhang, Xiao [1 ]
Dou, Wenchao [1 ]
Zhan, Xuejia [1 ]
Zhao, Guangying [1 ]
机构：
[1] Zhejiang Gongshang Univ, Dept Food Qual & Safety, Food Safety Key Lab Zhejiang Prov, Hangzhou 310035, Zhejiang, Peoples R China
来源：
ELECTROCHIMICA ACTA | 2012年 / 61卷
基金：
中国国家自然科学基金;
关键词：
Enterobacter sakazakii; Immunosensor; 1-Butyl-3-methylimida-zolium hexafluorophosphate; Multiwalled carbon nanotube; Thionine; DISPOSABLE ELECTROCHEMICAL IMMUNOSENSOR; SEPARATION-FREE IMMUNOASSAY; AMPEROMETRIC IMMUNOSENSOR; MULTILAYER FILMS; LIQUID; PCR; THIONINE; SALMONELLA;
D O I：
10.1016/j.electacta.2011.11.092
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A novel immunosensor for Enterobacter sakazakii (E. sakazakii) based on screen-printed carbon electrode (SPCE) modified by multiwalled carbon nanotube (MWCNT), 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF6) and thionine (Thi) was developed for the first time. The surface morphologies of modified electrodes were characterized by means of atomic force microscope (AFM). The electrochemical performance of the immunosensor was evaluated by cyclic voltammetry. Horseradish peroxidase labeled antibody to E. sakazakii in [BMIM[PF6 showed high activity and stability. Under optimal conditions, E. sakazakii could be detected in range of 10(3)-10(9) CFU/ml with detection limit of 7.7 x 10(1) CFU/ml. The proposed immunosensor exhibited excellent long-time storage stability and had potential use in clinical immunoassay of E. sakazakii. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：73 / 77
页数：5
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