Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9

被引：1
|
作者：
Huang, CJ [1 ]
Wang, TK [1 ]
Chung, SC [1 ]
Chen, CY [1 ]
机构：
[1] Natl Taiwan Univ, Dept Plant Pathol & Microbiol, Taipei 10764, Taiwan
来源：
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2005年 / 38卷 / 01期
关键词：
antifungal activity; chitinase; ChiCW; gene cloning; protein purification;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis; leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5alpha. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.
引用
收藏
页码：82 / 88
页数：7
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