Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9

被引：1

作者：

Huang, CJ ^{[1
]}

Wang, TK ^{[1
]}

Chung, SC ^{[1
]}

Chen, CY ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Plant Pathol & Microbiol, Taipei 10764, Taiwan

来源：

JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2005年 / 38卷 / 01期

关键词：

antifungal activity; chitinase; ChiCW; gene cloning; protein purification;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis; leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5alpha. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.

引用

页码：82 / 88

页数：7

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