1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

被引：19
|
作者：
Ravera, Enrico [1 ,2 ]
Cerofolini, Linda [1 ,2 ,3 ]
Martelli, Tommaso [4 ]
Louka, Alexandra [1 ,2 ]
Fragai, Marco [1 ,2 ,3 ]
Luchinat, Claudio [1 ,2 ,3 ,4 ]
机构：
[1] Univ Florence, Magnet Resonance Ctr CERM, Via L Sacconi 6, I-50019 Sesto Fiorentino, FI, Italy
[2] Interuniv Consortium Magnet Resonance Metalloprot, Via L Sacconi 6, I-50019 Sesto Fiorentino, FI, Italy
[3] Univ Florence, Dept Chem Ugo Schiff, Via Lastruccia 3, I-50019 Sesto Fiorentino, FI, Italy
[4] Giotto Biotech SRL, Via Madonna del Piano 6, I-50019 Sesto Fiorentino, FI, Italy
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
关键词：
ANGLE-SPINNING NMR; HIGH-RESOLUTION; CORRELATION SPECTROSCOPY; ENZYME IMMOBILIZATION; DECOUPLING SEQUENCES; BACKBONE ASSIGNMENT; DIATOM BIOSILICA; FUSION PROTEINS; BIOMINERALIZATION; PEPTIDES;
D O I：
10.1038/srep27851
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Proton-detection in solid-state NMR, enabled by high magnetic fields (>18 T) and fast magic angle spinning (>50 kHz), allows for the acquisition of traditional H-1-N-15 experiments on systems that are too big to be observed in solution. Among those, proteins entrapped in a bioinspired silica matrix are an attractive target that is receiving a large share of attention. We demonstrate that H-1-detected SSNMR provides a novel approach to the rapid assessment of structural integrity in proteins entrapped in bioinspired silica.
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页数：7
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