1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

被引：19

作者：

Ravera, Enrico ^{[1
,2
]}

Cerofolini, Linda ^{[1
,2
,3
]}

Martelli, Tommaso ^{[4
]}

Louka, Alexandra ^{[1
,2
]}

Fragai, Marco ^{[1
,2
,3
]}

Luchinat, Claudio ^{[1
,2
,3
,4
]}

机构：

[1] Univ Florence, Magnet Resonance Ctr CERM, Via L Sacconi 6, I-50019 Sesto Fiorentino, FI, Italy

[2] Interuniv Consortium Magnet Resonance Metalloprot, Via L Sacconi 6, I-50019 Sesto Fiorentino, FI, Italy

[3] Univ Florence, Dept Chem Ugo Schiff, Via Lastruccia 3, I-50019 Sesto Fiorentino, FI, Italy

[4] Giotto Biotech SRL, Via Madonna del Piano 6, I-50019 Sesto Fiorentino, FI, Italy

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

ANGLE-SPINNING NMR; HIGH-RESOLUTION; CORRELATION SPECTROSCOPY; ENZYME IMMOBILIZATION; DECOUPLING SEQUENCES; BACKBONE ASSIGNMENT; DIATOM BIOSILICA; FUSION PROTEINS; BIOMINERALIZATION; PEPTIDES;

D O I：

10.1038/srep27851

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Proton-detection in solid-state NMR, enabled by high magnetic fields (>18 T) and fast magic angle spinning (>50 kHz), allows for the acquisition of traditional H-1-N-15 experiments on systems that are too big to be observed in solution. Among those, proteins entrapped in a bioinspired silica matrix are an attractive target that is receiving a large share of attention. We demonstrate that H-1-detected SSNMR provides a novel approach to the rapid assessment of structural integrity in proteins entrapped in bioinspired silica.

引用

页数：7

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