In situ monitoring of chondrocyte response to bioactive scaffolds using Raman spectroscopy

被引：10
|
作者：
Jones, JR
Vats, A
Notingher, L
Gough, JE
Tolley, NS
Polak, JM
Hench, LL
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Mat, London SW7 2BP, England
[2] Univ London Imperial Coll Sci Technol & Med, Fac Med, Tissue Engn & Regenerat Med Ctr, Chelsea & Westminster Hosp, London SW10 9NH, England
[3] St Marys Hosp, London W2, England
[4] Univ Manchester, Manchester Mat Sci Ctr, Manchester M1 7HS, Lancs, England
[5] Univ Manchester, Manchester M1 7HS, Lancs, England
来源：
BIOCERAMICS 17 | 2005年 / 284-286卷
关键词：
bioactive glass; cartilage; chondrocytes; Raman spectroscopy; scaffolds;
D O I：
10.4028/www.scientific.net/KEM.284-286.623
中图分类号：
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Septal cartilage is widely used for the repair of soft tissue defects in the head, neck and nose. Tissue Engineering techniques are being investigated to create cartilage in vitro by seeding appropriate cells on resorbable scaffolds. In this study, human chondrocytes were cultured on macroporous bioactive glass foam scaffolds. The aim was to investigate how Raman spectroscopy could be used as a non-invasive technique to monitor the response of chondrocytes to a 3D scaffold in real time. The spectra were compared to scanning electron microscope (SEM) micrographs and immunohistochemistry results.
引用
收藏
页码：623 / 626
页数：4
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