Development of fluoroapatite chromatography for the purification of monoclonal antibody

被引：5

作者：

Ng, Paul K. ^{[1
]}

Yoshitake, Tomohiko ^{[2
]}

Snyder, Mark A. ^{[1
]}

机构：

[1] Biorad Labs, Life Sci Grp, Proc R&D Applicat, Hercules, CA 94547 USA

[2] HOYA Corp, PENTAX New Ceram Div, Tokyo, Japan

来源：

JOURNAL OF SEPARATION SCIENCE | 2010年 / 33卷 / 17-18期

关键词：

Antibody; Fluoroapatite chromatography; pH control; Protein A; Purity; PROTEIN; IMMUNOGLOBULIN;

D O I：

10.1002/jssc.201000335

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monoclonal antibody was purified from tissue culture fluid using ceramic fluoroapatite chromatography. Efficiency of the capture step was shown to be sensitive to pH and phosphate concentration. More than 90% of host cell proteins were removed by ceramic fluoroapatite chromatography. Studies regarding pH control and metal adsorption are presented.

引用

页码：2762 / 2767

页数：6

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