Development of fluoroapatite chromatography for the purification of monoclonal antibody

被引：5
|
作者：
Ng, Paul K. [1 ]
Yoshitake, Tomohiko [2 ]
Snyder, Mark A. [1 ]
机构：
[1] Biorad Labs, Life Sci Grp, Proc R&D Applicat, Hercules, CA 94547 USA
[2] HOYA Corp, PENTAX New Ceram Div, Tokyo, Japan
来源：
JOURNAL OF SEPARATION SCIENCE | 2010年 / 33卷 / 17-18期
关键词：
Antibody; Fluoroapatite chromatography; pH control; Protein A; Purity; PROTEIN; IMMUNOGLOBULIN;
D O I：
10.1002/jssc.201000335
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Monoclonal antibody was purified from tissue culture fluid using ceramic fluoroapatite chromatography. Efficiency of the capture step was shown to be sensitive to pH and phosphate concentration. More than 90% of host cell proteins were removed by ceramic fluoroapatite chromatography. Studies regarding pH control and metal adsorption are presented.
引用
收藏
页码：2762 / 2767
页数：6
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