Strategies for prokaryotic expression of eukaryotic membrane proteins

被引：45
|
作者：
Laage, R [1 ]
Langosch, D [1 ]
机构：
[1] Heidelberg Univ, Dept Neurol, D-69120 Heidelberg, Germany
来源：
TRAFFIC | 2001年 / 2卷 / 02期
关键词：
expression; synaptobrevin; syntaxin; SNARE; transmembrane segment; E; coli;
D O I：
10.1034/j.1600-0854.2001.020204.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcos aureus are presented.
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页码：99 / 104
页数：6
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