Determination of Lactose in Milk by Raman Spectroscopy

被引：20
|
作者：
Li, Meimei [1 ,2 ]
Chen, Jun [2 ]
Xu, Jiajie [1 ]
Fu, Shuilin [1 ]
Gong, Heng [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
[2] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
来源：
ANALYTICAL LETTERS | 2015年 / 48卷 / 08期
基金：
中国国家自然科学基金;
关键词：
Raman spectroscopy; Lactose; Milk; AMPEROMETRIC BIOSENSOR; GLUCOSE; PERFORMANCE; SCATTERING; PROTEIN;
D O I：
10.1080/00032719.2014.979358
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A Raman spectroscopy-based approach was developed for the rapid determination of lactose in milk using crystal violet as an internal standard. The crystal violet peak at 1173 reciprocal centimeters did not interfere with the lactose peak at 1085 reciprocal centimeters; therefore, crystal violet can be used to quantify lactose in milk. This developed method allowed the determination of lactose from 0.028-0.1mole per liter, with a limit of detection of 0.019mole per liter. The results revealed that Raman spectroscopy is a simple and rapid method for the determination of lactose in milk.
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页码：1333 / 1340
页数：8
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