Biochemical Characterization of the Subclass B3 Metallo-β-Lactamase PJM-1 from Pseudoxanthomonas japonensis

被引：3

作者：

Yamada, Kageto ^{[1
]}

Ishii, Yoshikazu ^{[1
]}

Tateda, Kazuhiro ^{[1
]}

机构：

[1] Toho Univ, Dept Microbiol & Infect Dis, Sch Med, Ota Ku, Tokyo, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2022年 / 66卷 / 09期

关键词：

MBL; PJM-1; Pseudoxanthomonas japonensis; PSEUDOMONAS-AERUGINOSA; SP-NOV;

D O I：

10.1128/aac.00691-22

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Biochemical properties of the novel subclass B3 metallo-beta-lactamase (MBL) PJM-1 expressed in Pseudoxanthomonas japonensis, which is often isolated from the environment, were determined. The 906-bp bla(PJM-1) gene in P. japonensis is a species-specific MBL gene, and PJM, with 301 predicted amino acids, has 81.8% amino acid identity with AIM-1. In this study, PJM-1 was recombinantly expressed and purified. PJM-1 showed a low catalytic activity against ceftazidime and cefepime, and it was strongly inhibited by EDTA.

引用

页数：5

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