Biochemical Characterization of the Subclass B3 Metallo-β-Lactamase PJM-1 from Pseudoxanthomonas japonensis

被引：3

作者：

Yamada, Kageto ^{[1
]}

Ishii, Yoshikazu ^{[1
]}

Tateda, Kazuhiro ^{[1
]}

机构：

[1] Toho Univ, Dept Microbiol & Infect Dis, Sch Med, Ota Ku, Tokyo, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2022年 / 66卷 / 09期

关键词：

MBL; PJM-1; Pseudoxanthomonas japonensis; PSEUDOMONAS-AERUGINOSA; SP-NOV;

D O I：

10.1128/aac.00691-22

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Biochemical properties of the novel subclass B3 metallo-beta-lactamase (MBL) PJM-1 expressed in Pseudoxanthomonas japonensis, which is often isolated from the environment, were determined. The 906-bp bla(PJM-1) gene in P. japonensis is a species-specific MBL gene, and PJM, with 301 predicted amino acids, has 81.8% amino acid identity with AIM-1. In this study, PJM-1 was recombinantly expressed and purified. PJM-1 showed a low catalytic activity against ceftazidime and cefepime, and it was strongly inhibited by EDTA.

引用

页数：5

共 50 条

[21] QM/MM investigation of substrate binding of subclass B3 metallo-β-lactamase SMB-1 from Serratia marcescents: insights into catalytic mechanism
Mu, Xia
Xu, Dingguo
JOURNAL OF MOLECULAR MODELING, 2020, 26 (04)
[22] Genetic and biochemical characterization of HMB-1, a novel subclass B1 metallo-β-lactamase found in a Pseudomonas aeruginosa clinical isolate
Pfennigwerth, Niels
Lange, Felix
Campos, Cristina Belmar
Hentschke, Moritz
Gatermann, Soeren G.
Kaase, Martin
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2017, 72 (04) : 1068 - 1073
[23] QM/MM investigation of substrate binding of subclass B3 metallo-β-lactamase SMB-1 from Serratia marcescents: insights into catalytic mechanism
Xia Mu
Dingguo Xu
Journal of Molecular Modeling, 2020, 26
[24] Biochemical Characterization of Sfh-I, a Subclass B2 Metallo-β-Lactamase from Serratia fonticola UTAD54
Fonseca, Fatima
Arthur, Christopher J.
Bromley, Elizabeth H. C.
Samyn, Bart
Moerman, Pablo
Saavedra, Maria Jose
Correia, Antonio
Spencer, James
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (11) : 5392 - 5395
[25] Biochemical characterization of the acquired metallo-β-lactamase SPM-1 from Pseudomonas aeruginosa
Murphy, TA
Simm, AM
Toleman, MA
Jones, RN
Walsh, TR
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (02) : 582 - 587
[26] Biochemical characterization of the L1-like metallo-β-lactamase from Stenotrophomonas lactitubi
Yamada, Kageto
Ishii, Yoshikazu
Tateda, Kazuhiro
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2024, 68 (03)
[27] Kinetic and Structural Characterization of the First B3 Metallo-β-Lactamase with an Active-Site Glutamic Acid
Wilson, Liam A.
Knaven, Esmee G.
Morris, Marc T.
Pedroso, Marcelo Monteiro
Schofield, Christopher J.
Brueck, Thomas B.
Boden, Mikael
Waite, David W.
Hugenholtz, Philip
Guddat, Luke
Schenk, Gerhard
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2021, 65 (10)
[28] Genetic and Biochemical Characterization of an Acquired Subgroup B3 Metallo-β-Lactamase Gene, blaAIM-1, and Its Unique Genetic Context in Pseudomonas aeruginosa from Australia
Yong, Dongeun
Toleman, Mark A.
Bell, Jan
Ritchie, Brett
Pratt, Rachael
Ryley, Henry
Walsh, Timothy R.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (12) : 6154 - 6159
[29] The Legionella (Fluoribacter) gormanii metallo-β-lactamase:: a new member of the highly divergent lineage of molecular-subclass B3 β-lactamases
Boschi, L
Mercuri, PS
Riccio, ML
Amicosante, G
Galleni, M
Frère, JM
Rossolini, GM
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (06) : 1538 - 1543
[30] High-Resolution Crystal Structure of the Subclass B3 Metallo-β-Lactamase BJP-1: Rational Basis for Substrate Specificity and Interaction with Sulfonamides
Docquier, Jean-Denis
Benvenuti, Manuela
Calderone, Vito
Stoczko, Magdalena
Menciassi, Nicola
Rossolini, Gian Maria
Mangani, Stefano
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (10) : 4343 - 4351

← 1 2 3 4 5 →