Biochemical Characterization of the Subclass B3 Metallo-β-Lactamase PJM-1 from Pseudoxanthomonas japonensis

被引：3
|
作者：
Yamada, Kageto [1 ]
Ishii, Yoshikazu [1 ]
Tateda, Kazuhiro [1 ]
机构：
[1] Toho Univ, Dept Microbiol & Infect Dis, Sch Med, Ota Ku, Tokyo, Japan
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2022年 / 66卷 / 09期
关键词：
MBL; PJM-1; Pseudoxanthomonas japonensis; PSEUDOMONAS-AERUGINOSA; SP-NOV;
D O I：
10.1128/aac.00691-22
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Biochemical properties of the novel subclass B3 metallo-beta-lactamase (MBL) PJM-1 expressed in Pseudoxanthomonas japonensis, which is often isolated from the environment, were determined. The 906-bp bla(PJM-1) gene in P. japonensis is a species-specific MBL gene, and PJM, with 301 predicted amino acids, has 81.8% amino acid identity with AIM-1. In this study, PJM-1 was recombinantly expressed and purified. PJM-1 showed a low catalytic activity against ceftazidime and cefepime, and it was strongly inhibited by EDTA.
引用
收藏
页数：5
相关论文
共 50 条
  • [1] Biochemical Characterization of CPS-1, a Subclass B3 Metallo-β-Lactamase from a Chryseobacterium piscium Soil Isolate
    Gudeta, Dereje Dadi
    Pollini, Simona
    Docquier, Jean-Denis
    Bortolaia, Valeria
    Rossolini, Gian Maria
    Guardabassi, Luca
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (03) : 1869 - 1873
  • [2] Structural and Biochemical Characterization of Rm3, a Subclass B3 Metallo-β-Lactamase Identified from a Functional Metagenomic Study
    Salimraj, Ramya
    Zhang, Lihong
    Hinchliffe, Philip
    Wellington, Elizabeth M. H.
    Brem, Juergen
    Schofield, Christopher J.
    Gaze, William H.
    Spencer, James
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (10) : 5828 - 5840
  • [3] Structural Insights into the Subclass B3 Metallo-β-Lactamase SMB-1 and the Mode of Inhibition by the Common Metallo-β-Lactamase Inhibitor Mercaptoacetate
    Wachino, Jun-ichi
    Yamaguchi, Yoshihiro
    Mori, Shigetarou
    Kurosaki, Hiromasa
    Arakawa, Yoshichika
    Shibayama, Keigo
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (01) : 101 - 109
  • [4] Biochemical and Structural Characterization of the Subclass B1 Metallo-β-Lactamase VIM-4
    Lassaux, Patricia
    Traore, Daouda A. K.
    Loisel, Elodie
    Favier, Adrien
    Docquier, Jean-Denis
    Sohier, Jean Sebastien
    Laurent, Clementine
    Bebrone, Carine
    Frere, Jean-Marie
    Ferrer, Jean-Luc
    Galleni, Moreno
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (03) : 1248 - 1255
  • [5] Genetic, biochemical, and structural analyses of a subclass B3 metallo-β-lactamase SMB-1 from a Serratia marcescens clinical isolate
    Wachino, J. -I.
    Arakawa, Y.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2013, 42 : S55 - S56
  • [6] Characterization of NWM-1, a novel subclass B3 metallo-β-lactamase found in a clinical isolate of Pseudomonas aeruginosa
    Hoefken, Lisa-Marie
    Schauer, Jennifer
    Eisfeld, Jessica
    Dziobaka, Jan
    Gatermann, Soeren G.
    Pfennigwerth, Niels
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2024,
  • [7] PNGM-1, a novel subclass B3 metallo-β-lactamase from a deep-sea sediment metagenome
    Park, Kwang Seung
    Kim, Tae Yeong
    Kim, Ji Hwan
    Lee, Jung Hun
    Jeon, Jeong Ho
    Karim, Asad Mustafa
    Malik, Sumera Kausar
    Lee, Sang Hee
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2018, 14 : 302 - 305
  • [8] Postgenomic scan of metallo-β-lactamase homologues in rhizobacteria:: Identification and characterization of BJP-1, a subclass B3 ortholog from Bradyrhizobium japonicum
    Stoczko, Magdalena
    Frere, Jean-Marie
    Rossolini, Gian Maria
    Docquier, Jean-Denis
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (06) : 1973 - 1981
  • [9] Structural Insights into Recognition of Hydrolyzed Carbapenems and Inhibitors by Subclass B3 Metallo-β-Lactamase SMB-1
    Wachino, Jun-ichi
    Yamaguchi, Yoshihiro
    Mori, Shigetarou
    Jin, Wanchun
    Kimura, Kouji
    Kurosaki, Hiromasa
    Arakawa, Yoshichika
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (07) : 4274 - 4282
  • [10] 4-Amino-2-Sulfanylbenzoic Acid as a Potent Subclass B3 Metallo-β-Lactamase-Specific Inhibitor Applicable for Distinguishing Metallo-β-Lactamase Subclasses
    Wachino, Jun-ichi
    Kanechi, Reo
    Nishino, Erina
    Mochizuki, Marie
    Jin, Wanchun
    Kimura, Kouji
    Kurosaki, Hiromasa
    Arakawa, Yoshichika
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2019, 63 (10)
12345