Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy

被引：0
|
作者：
Chen, TS [1 ]
Liu, YF [1 ]
Zeng, SQ [1 ]
Luo, QM [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Minist Educ, Key Lab Biomed Photon, Wuhan 430074, Peoples R China
来源：
PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE | 2003年 / 5254卷
关键词：
photobleaching rate; two-photon excitation; rhodamine B;
D O I：
10.1117/12.546559
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The intensity-dependence of photobleaching rate with two-photon excitation (TPE) at biological imaging level has been found to be high-order (>3) relationship. This paper studied experimentally the dependence for rhodamine B (RhB) at two different concentrations under unsaturated absorption conditions. The log-log plot of photobleaching rate versus incident power at concentration of 10(-2)M increased with a slope of similar to3.7, but that at concentration of 10(-4) M increased with a slope of similar to2.7.
引用
收藏
页码：508 / 512
页数：5
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