Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy

被引：0
|
作者：
Chen, TS [1 ]
Liu, YF [1 ]
Zeng, SQ [1 ]
Luo, QM [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Minist Educ, Key Lab Biomed Photon, Wuhan 430074, Peoples R China
来源：
PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE | 2003年 / 5254卷
关键词：
photobleaching rate; two-photon excitation; rhodamine B;
D O I：
10.1117/12.546559
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The intensity-dependence of photobleaching rate with two-photon excitation (TPE) at biological imaging level has been found to be high-order (>3) relationship. This paper studied experimentally the dependence for rhodamine B (RhB) at two different concentrations under unsaturated absorption conditions. The log-log plot of photobleaching rate versus incident power at concentration of 10(-2)M increased with a slope of similar to3.7, but that at concentration of 10(-4) M increased with a slope of similar to2.7.
引用
收藏
页码：508 / 512
页数：5
相关论文
共 50 条
  • [21] Two-photon excitation microscopy for analyses of biofilm processes
    Bryers, JD
    MICROBIAL GROWTH IN BIOFILMS, PT B: SPECIAL ENVIRONMENTS AND PHYSICOCHEMICAL ASPECTS, 2001, 337 : 259 - 269
  • [22] Two-photon excitation microscopy with spatial light modulator
    Matsumoto, Naoya
    Konno, Alu
    Inoue, Takashi
    Toyoda, Haruyoshi
    Miwa, Toshiyuki
    Nakamura, Kazuhiro
    Okazaki, Shigetoshi
    BIOMEDICAL IMAGING AND SENSING CONFERENCE, 2017, 10251
  • [23] Pulse compression in two-photon excitation fluorescence microscopy
    Liang, Xiaobao
    Hu, Wenyan
    Fu, Ling
    OPTICS EXPRESS, 2010, 18 (14): : 14893 - 14904
  • [24] Combined optical coherence and two-photon excitation microscopy
    Beaurepaire, E
    Moreaux, L
    Mertz, J
    PROCEEDINGS OF INTER-INSTITUTE WORKSHOP ON IN VIVO OPTICAL IMAGING AT THE NIH, 2000, : 43 - 48
  • [25] Antecedents of two-photon excitation laser scanning microscopy
    Masters, BR
    So, PTC
    MICROSCOPY RESEARCH AND TECHNIQUE, 2004, 63 (01) : 3 - 11
  • [26] Two-photon excitation STED-CW microscopy
    Bianchini, Paolo
    Harke, Benjamin
    Galiani, Silvia
    Diaspro, Alberto
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XI, 2011, 7903
  • [27] Quantitative imaging of metabolism by two-photon excitation microscopy
    Piston, DW
    Knobel, SM
    CONFOCAL MICROSCOPY, 1999, 307 : 351 - 368
  • [28] Continuous wave excitation two-photon fluorescence microscopy
    Hanninen, P.E.
    Soini, E.
    Hell, S.W.
    Journal of Microscopy, 1994, 176 (03) : 222 - 225
  • [29] Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants
    Stoneman, Michael R.
    McCoy, Victoria E.
    Gee, Carole T.
    Bober, Katherine M. M.
    Raicu, Valerica
    COMMUNICATIONS BIOLOGY, 2024, 7 (01)
  • [30] Photobleaching analysis of fluorescent proteins in two-photon microscopy at high repetition rates
    Weng, Chun Hung
    Han, Kyu Young
    OPTICS COMMUNICATIONS, 2025, 574
12345