High-throughput sequencing and clinical microbiology: progress, opportunities and challenges

被引：105

作者：

Pallen, Mark J. ^{[1
]}

Loman, Nicholas J. ^{[1
]}

Penn, Charles W. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

CURRENT OPINION IN MICROBIOLOGY | 2010年 / 13卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

BACTERIAL; COMPLEXITY; DIVERSITY; EVOLUTION; MUTANTS; CATALOG; CELLS;

D O I：

10.1016/j.mib.2010.08.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

High-throughput sequencing is sweeping through clinical microbiology, transforming our discipline in its wake. It is already providing an enhanced view of pathogen biology through rapid and inexpensive whole-genome sequencing and more sophisticated applications such as RNA-seq. It also promises to deliver high-resolution genomic epidemiology as the ultimate typing method for bacteria. However, the most revolutionary effect of this 'disruptive technology' is likely to be creation of a novel sequence-based, culture-independent diagnostic microbiology that incorporates microbial community profiling, metagenomics and single-cell genomics. We should prepare for the coming 'technological singularity' in sequencing, when this technology becomes so fast and so cheap that it threatens to out-compete existing diagnostic and typing methods in microbiology.

引用

页码：625 / 631

页数：7

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