lncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head

被引:3
|
作者
Wu, Yadi [1 ]
Fang, Lun [1 ]
Gao, Yong [2 ]
Zhao, Zhiqiu [1 ]
Zhou, Lu [3 ]
Zhang, Gang [2 ]
机构
[1] Shandong First Med Univ, Shandong Acad Med Sci, Tai An 271016, Shandong, Peoples R China
[2] Shandong First Med Univ, Dept Orthoped, Affiliated Hosp 2, Tai An 271000, Shandong, Peoples R China
[3] Shandong First Med Univ, Shandong Acad Med Sci, Inst Sports Med, Tai An 271016, Shandong, Peoples R China
关键词
NONTRAUMATIC OSTEONECROSIS; DIFFERENTIATION; CANCER; PROGRESSION;
D O I
10.1155/2022/9364467
中图分类号
R19 [保健组织与事业(卫生事业管理)];
学科分类号
摘要
Background. Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. This study aims to clarify the regulatory mechanism of lncRNA FGD5-AS1 in ONFH. Methods. The expression of the protein and mRNA was detected by RT-qPCR and Western blot assay. The regulatory mechanism of lncRNA FGD5-AS1 was detected by the dual-luciferase reporter assay, CCK-8 assay, and flow cytometry assay. Results. Dex can inhibit cell proliferation and differentiation and induce apoptosis in hBMSCs in a dose-dependent manner. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. Also, miR-296-5p directly targets STAT3. More importantly, miR-296-5p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs. Conclusion. lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3.
引用
收藏
页数:9
相关论文
共 50 条
  • [31] CircHIPK3 promotes bone microvascular endothelial cell proliferation, migration and angiogenesis by targeting miR-7 and KLF4/VEGF signaling in steroid-induced osteonecrosis of the femoral head
    Peng, Peng
    He, Wei
    Zhang, Yi-Xi
    Liu, Xiao-Hua
    Chen, Zhen-Qiu
    Mao, Ji-Gang
    ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE, 2023, 32 (01): : 43 - 55
  • [32] A novel LncRNA SPIRE1/miR-181a-5p/PRLR axis in mandibular bone marrow-derived mesenchymal stem cells regulates the Th17/Treg immune balance through the JAK/STAT3 pathway in periodontitis
    Shao, Bingyi
    Zhou, Duo
    Wang, Jie
    Yang, Deqin
    Gao, Jing
    AGING-US, 2023, 15 (14): : 7124 - 7145
  • [33] STAT3-induced lncRNA GNAS-AS1 accelerates keloid formation by mediating the miR-196a-5p/CXCL12/STAT3 axis in a feedback loop
    Liu, Yun
    Ma, Teng-Xiao
    Fan, Peng-Fei
    Wang, Ze
    Wang, Zhe
    Li, Lei
    EXPERIMENTAL DERMATOLOGY, 2024, 33 (06)
  • [34] CircPVT1 up-regulation attenuates steroid-induced osteonecrosis of the femoral head through regulating miR-21-5p-mediated Smad7/TGFβ signalling pathway
    Hao, Yangquan
    Lu, Chao
    Zhang, Baogang
    Xu, Zhaochen
    Guo, Hao
    Zhang, Gaokui
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021, 25 (10) : 4608 - 4622
  • [35] SP1-induced lncRNA TUG1 regulates proliferation and apoptosis in islet cells of type 2 diabetes mellitus via the miR-188-3p/FGF5 axis
    Zhang, P.
    Li, Y-N
    Tu, S.
    Cheng, X-B
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2021, 25 (04) : 1959 - 1966
  • [36] RETRACTED: DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis (Retracted article. See vol. 15, pg. 771, 2023)
    Ma, Chuanyu
    Ma, Ning
    Qin, Lili
    Miao, Chuanna
    Luo, Minglei
    Liu, Shuhong
    CANCER MANAGEMENT AND RESEARCH, 2020, 12 : 10035 - 10046
  • [37] LncRNA RPSAP52 regulates miR-423-5p/GSTM1 axis to suppress hypoxia-induced renal proximal tubular epithelial cell apoptosis
    Chen, Jie
    Zheng, Yu
    Li, Liping
    ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, 2022, 128 (04) : 1066 - 1070
  • [38] Bone marrow mesenchymal stem cells-derived exosomes mediate nuclear receptor coactivator-3 expression in osteoblasts by delivering miR-532-5p to influence osteonecrosis of the femoral head development
    Lan, Xiaoyong
    Ma, Haiping
    Xiong, Yipin
    Zou, Lingfeng
    Yuan, Zhen
    Xiao, Yuhong
    CELL BIOLOGY INTERNATIONAL, 2022, 46 (12) : 2185 - 2197
  • [39] LncRNA FOXD1-AS1 regulates pancreatic cancer stem cell properties and 5-FU resistance by regulating the miR-570-3p/SPP1 axis as a ceRNA
    Liu Ouyang
    Min-min Sun
    Ping-sheng Zhou
    Yi-wei Ren
    Xin-yu Liu
    Wan-ying Wei
    Zhen-shun Song
    Kai Lu
    Li-xue Yang
    Cancer Cell International, 24
  • [40] LncRNA FOXD1-AS1 regulates pancreatic cancer stem cell properties and 5-FU resistance by regulating the miR-570-3p/SPP1 axis as a ceRNA
    Ouyang, Liu
    Sun, Min-min
    Zhou, Ping-sheng
    Ren, Yi-wei
    Liu, Xin-yu
    Wei, Wan-ying
    Song, Zhen-shun
    Lu, Kai
    Yang, Li-xue
    CANCER CELL INTERNATIONAL, 2024, 24 (01)