lncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head

被引:3
|
作者
Wu, Yadi [1 ]
Fang, Lun [1 ]
Gao, Yong [2 ]
Zhao, Zhiqiu [1 ]
Zhou, Lu [3 ]
Zhang, Gang [2 ]
机构
[1] Shandong First Med Univ, Shandong Acad Med Sci, Tai An 271016, Shandong, Peoples R China
[2] Shandong First Med Univ, Dept Orthoped, Affiliated Hosp 2, Tai An 271000, Shandong, Peoples R China
[3] Shandong First Med Univ, Shandong Acad Med Sci, Inst Sports Med, Tai An 271016, Shandong, Peoples R China
关键词
NONTRAUMATIC OSTEONECROSIS; DIFFERENTIATION; CANCER; PROGRESSION;
D O I
10.1155/2022/9364467
中图分类号
R19 [保健组织与事业(卫生事业管理)];
学科分类号
摘要
Background. Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. This study aims to clarify the regulatory mechanism of lncRNA FGD5-AS1 in ONFH. Methods. The expression of the protein and mRNA was detected by RT-qPCR and Western blot assay. The regulatory mechanism of lncRNA FGD5-AS1 was detected by the dual-luciferase reporter assay, CCK-8 assay, and flow cytometry assay. Results. Dex can inhibit cell proliferation and differentiation and induce apoptosis in hBMSCs in a dose-dependent manner. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. Also, miR-296-5p directly targets STAT3. More importantly, miR-296-5p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs. Conclusion. lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3.
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页数:9
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