Catalyst-Free Conjugation and In Situ Quantification of Nanoparticle Ligand Surface Density Using Fluorogenic Cu-Free Click Chemistry

被引：10
|
作者：
Jolck, Rasmus I. [1 ]
Sun, Honghao [1 ]
Berg, Rolf H. [1 ]
Andresen, Thomas L. [1 ]
机构：
[1] Tech Univ Denmark, Dept Micro & Nanotechnol, DK-4000 Roskilde, Denmark
来源：
CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 12期
关键词：
click chemistry; coumarin; liposomes; nanoparticles; surface functionalization; 1,3-DIPOLAR CYCLOADDITION; TERMINAL ALKYNES; LIPOSOMES; PEPTIDE; CANCER; AZIDES; STRATEGIES; LIGATION; DESIGN; CELLS;
D O I：
10.1002/chem.201003131
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency and ligand surface density has been developed. Attachment of 3-azido-modifed RGD-peptides to PEGylated liposomes was achieved by using Cu-free click conditions. Upon coupling a fluorophore is formed, which could be utilized to monitor conjugation efficiency and the obtained ligand surface density in situ, without prior purification. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：3326 / 3331
页数：6
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