Catalyst-Free Conjugation and In Situ Quantification of Nanoparticle Ligand Surface Density Using Fluorogenic Cu-Free Click Chemistry

被引：10

作者：

Jolck, Rasmus I. ^{[1
]}

Sun, Honghao ^{[1
]}

Berg, Rolf H. ^{[1
]}

Andresen, Thomas L. ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Micro & Nanotechnol, DK-4000 Roskilde, Denmark

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 12期

关键词：

click chemistry; coumarin; liposomes; nanoparticles; surface functionalization; 1,3-DIPOLAR CYCLOADDITION; TERMINAL ALKYNES; LIPOSOMES; PEPTIDE; CANCER; AZIDES; STRATEGIES; LIGATION; DESIGN; CELLS;

D O I：

10.1002/chem.201003131

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency and ligand surface density has been developed. Attachment of 3-azido-modifed RGD-peptides to PEGylated liposomes was achieved by using Cu-free click conditions. Upon coupling a fluorophore is formed, which could be utilized to monitor conjugation efficiency and the obtained ligand surface density in situ, without prior purification. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：3326 / 3331

页数：6

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