Platelet release of β-thromboglobulin and platelet factor 4 and serotonin in plasma samples

被引：37
|
作者：
Ohkawa, R
Hirowatari, Y
Nakamura, K
Ohkubo, S
Ikeda, H
Okada, M
Tozuka, M
Nakahara, K
Yatomi, Y
机构：
[1] Univ Tokyo, Grad Sch Med, Dept Lab Med, Bunkyo Ku, Tokyo 1138655, Japan
[2] Tokyo Univ Hosp, Dept Clin Lab, Tokyo 113, Japan
[3] Tosoh Corp, Sci Instruments Div, Kanagawa, Japan
[4] Toho Univ, Dept Biomol Sci, Chiba 2748510, Japan
来源：
CLINICAL BIOCHEMISTRY | 2005年 / 38卷 / 11期
关键词：
plasma; platelet factor 4 (PF4); serotonin; beta-thromboglobulin (beta-TG);
D O I：
10.1016/j.clinbiochem.2005.07.008
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: Platelet release of a granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated. Methods: Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method. Results: beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin. Conclusions: Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples. (C) 2005 The Canadian Society of Clinical Chemists. All rights reserved.
引用
收藏
页码：1023 / 1026
页数：4
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