Platelet release of β-thromboglobulin and platelet factor 4 and serotonin in plasma samples

被引：37

作者：

Ohkawa, R

Hirowatari, Y

Nakamura, K

Ohkubo, S

Ikeda, H

Okada, M

Tozuka, M

Nakahara, K

Yatomi, Y

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Lab Med, Bunkyo Ku, Tokyo 1138655, Japan

[2] Tokyo Univ Hosp, Dept Clin Lab, Tokyo 113, Japan

[3] Tosoh Corp, Sci Instruments Div, Kanagawa, Japan

[4] Toho Univ, Dept Biomol Sci, Chiba 2748510, Japan

来源：

CLINICAL BIOCHEMISTRY | 2005年 / 38卷 / 11期

关键词：

plasma; platelet factor 4 (PF4); serotonin; beta-thromboglobulin (beta-TG);

D O I：

10.1016/j.clinbiochem.2005.07.008

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: Platelet release of a granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated. Methods: Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method. Results: beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin. Conclusions: Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples. (C) 2005 The Canadian Society of Clinical Chemists. All rights reserved.

引用

页码：1023 / 1026

页数：4

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