Research of the absorbance detection and fluorescence detection for multifunctional nutrition analyzer

The research of the multifunctional analyzer which integrates absorbance detection, fluorescence detection, time-resolved fluorescence detection, biochemical luminescence detection methods, can make efficient detection and analysis for a variety of human body nutrients. This article focuses on the absorbance detection and fluorescence detection system. The two systems are modular in design and controlled by embedded system, to achieve automatic measurement according to user settings. In the optical path design, the application of confocal design can improve the optical signal acquisition capability, and reduce the interference. A photon counter is used for detection, and a high performance counter module is designed to measure the output of photon counter. In the experiment, we use neutral density filters and potassium dichromate solution to test the absorbance detection system, and use fluorescein isothiocyanate(FITC) for fluorescence detection system performance test. The experimental results show that the absorbance detection system has a detection range of 0 similar to 4OD, and has good linearity in the detection range, while the fluorescence detection system has a high sensitivity of 1pmol/L concentration.