Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis

被引：22
|
作者：
de Moura, Tiane Martin [1 ,2 ]
Vaz Cassenego, Ana Paula [1 ,2 ]
Campos, Fabricio Souza [3 ,4 ]
Leal Ribeiro, Andrea Machado [5 ]
Franco, Ana Claudia [1 ,3 ]
d'Azevedo, Pedro Alves [7 ]
Frazzon, Jeverson [6 ]
Guedes Frazzon, Ana Paula [1 ,2 ]
机构：
[1] Univ Fed Rio Grande do Sul, Programa Posgrad Microbiol Agr & Ambiente, Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Bacteriol Lab, Dept Microbiol, Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Virol Lab, Dept Microbiol, Inst Ciencias Basicas Saude, Porto Alegre, RS, Brazil
[4] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Vet, Porto Alegre, RS, Brazil
[5] Univ Fed Rio Grande do Sul, Fac Agron, Dept Zootecnia, Porto Alegre, RS, Brazil
[6] Univ Fed Rio Grande do Sul, Inst Ciencia & Tecnol Alimentos, Porto Alegre, RS, Brazil
[7] Univ Fed Ciencias Saude Porto Alegre, Lab Cocos Gram Posit, Porto Alegre, RS, Brazil
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2013年 / 108卷 / 04期
关键词：
Enterococcus faecalis; vancomycin resistance; vanC gene; horizontal gene transfer; MULTIPLEX PCR; VANC-2/3; GENES; SPECIES LEVEL; D-ALANINE; RESISTANCE; IDENTIFICATION; FOOD; CASSELIFLAVUS; GALLINARUM; SEQUENCE;
D O I：
10.1590/S0074-0276108042013009
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Here we report the presence and expression levels of the vanC(1) and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC(1) and vanC2/3 genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC(1) gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.
引用
收藏
页码：453 / 456
页数：4
相关论文
共 50 条
  • [41] Detection of virulence genes in aminoglycoside susceptible and resistant Enterococcus faecalis
    Silva, Juan
    Rodriguez, Yara
    Araya, Jorge
    Gahona, Joselyne
    Valenzuela, Nicomedes
    Guerrero, Katheryne
    Baez, John
    Baquero, Fernando
    del Campo, Rosa
    REVISTA CHILENA DE INFECTOLOGIA, 2013, 30 (01): : 17 - 22
  • [42] COMPARISON OF THE IN-VITRO ACTIVITY OF LEVOFLOXACIN AND OTHER ANTIMICROBIAL AGENTS AGAINST VANCOMYCIN-SUSCEPTIBLE AND VANCOMYCIN-RESISTANT ENTEROCOCCUS SPECIES
    HAYDEN, MK
    MATUSHEK, MG
    TRENHOLME, GM
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1995, 22 (04) : 349 - 352
  • [43] Failure of daptomycin monotherapy for endocarditis caused by an Enterococcus faecium strain with vancomycin-resistant and vancomycin-susceptible subpopulations and evidence of in vivo loss of the vanA gene cluster
    Arias, Cesar A.
    Torres, Harrys A.
    Singh, Kavindra V.
    Panesso, Diana
    Moore, Judson
    Wanger, Audrey
    Murray, Barbara E.
    CLINICAL INFECTIOUS DISEASES, 2007, 45 (10) : 1343 - 1346
  • [44] Genetic Diversity of the Low-Level Vancomycin Resistance Gene vanC-2/vanC-3 and Identification of a Novel vanC Subtype (vanC-4) in Enterococcus casseliflavus
    Watanabe, Shojiro
    Kobayashi, Nobumichi
    Quinones, Dianelys
    Hayakawa, Satoshi
    Nagashima, Shigeo
    Uehara, Nobuyuki
    Watanabe, Naoki
    MICROBIAL DRUG RESISTANCE, 2009, 15 (01) : 1 - 9
  • [45] Novel chromogenic medium for detection of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis
    Cuzon, Gaelle
    Naas, Thierry
    Fortineau, Nicolas
    Nordmann, Patrice
    JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (07) : 2442 - 2444
  • [46] A novel chromogenic medium for vancomycin-resistant Enterococcus faecium and Enterococcus faecalis detection
    Cuzon, G.
    Naas, T.
    Fortineau, N.
    Nordmann, P.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2007, 29 : S22 - S22
  • [47] Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer
    Viera, CT
    Tsiodras, S
    Gold, HS
    Coakley, EPG
    Wennersten, C
    Eliopoulos, GM
    Moellering, RC
    Inouye, RT
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (03) : 973 - 975
  • [48] In vitro synergism and anti-biofilm activity of dalbavancin antibiotic combinations against vancomycin-susceptible and vancomycin-resistant Enterococcus faecium
    Thieme, L.
    Hartung, A.
    Makarewicz, O.
    Pletz, M. W.
    INFECTION, 2019, 47 : S12 - S13
  • [49] Characterization of a vancomycin-resistant Enterococcus faecium isolate and a vancomycin-susceptible E. faecium isolate from the same blood culture
    Xanthopoulou, Kyriaki
    Wille, Julia
    Zweigner, Janine
    Lucassen, Kai
    Wille, Thorsten
    Seifert, Harald
    Higgins, Paul G.
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2021, 76 (04) : 883 - 886
  • [50] PCR detection of the vanA gene in a vancomycin-resistant Enterococcus faecalis clinical isolate from Hungary
    Ghidán, A
    Jeney, C
    Maródi, CL
    Csiszár, K
    Rozgonyi, F
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (02) : 325 - 327
12345