High total TGF-beta 2 levels in normal human tears

被引：57
|
作者：
Kokawa, N
Sotozono, C
Nishida, K
Kinoshita, S
机构：
[1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602, Hirokoji Kawaramachi
来源：
CURRENT EYE RESEARCH | 1996年 / 15卷 / 03期
关键词：
active TGF-beta 2; ELISA; human tears; total TGF-beta 2;
D O I：
10.3109/02713689609007630
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Mature and total TGF-beta 2 levels were determined in normal human tears, from 37 volunteers, using a quantitative 'sandwich' enzyme immunoassay technique. Since this ELISA method recognizes only mature TGF-beta 2, total TGF-beta 2 levels in tears were measured after acid activation. Aqueous humor samples from 9 patients with cataract were also tested as controls. Mature and total TGF-beta 2 in aqueous humor (AH) averaged 182 pg/ml and 737 pg/ml, respectively. The levels of mature TGF-beta 2 in normal human tears ranged from 36 to 71 pg/ml (mean: 55 pg/ml). And the levels of total TGF-beta 2 ranged from 3446 to 14910 pg/ml (mean: 7854 pg/ml), a very high level. Percentage of mature TGF-beta 2 ranged 0.4 to 1.3% (mean: 0.9%). It is reported for the first time that there are very high levels of total TGF-beta 2 in normal human tears.
引用
收藏
页码：341 / 343
页数：3
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