Synthesis of Homo- and Heterofunctionalized Glycoclusters and Binding to Pseudomonas aeruginosa Lectins PA-IL and PA-IIL

被引：29

作者：

Gerland, Beatrice ^{[2
]}

Goudot, Alice ^{[1
]}

Pourceau, Gwladys ^{[2
]}

Meyer, Albert ^{[2
]}

Vidal, Sebastien ^{[3
]}

Souteyrand, Eliane ^{[1
]}

Vasseur, Jean-Jacques ^{[2
]}

Chevolot, Yann ^{[1
]}

Morvan, Francois ^{[2
]}

机构：

[1] Univ Lyon, Ecole Cent Lyon, CNRS, INL,UMR 5270, F-69134 Ecully, France

[2] Univ Montpellier 2, Univ Montpellier 1, CNRS, IBMM,UMR 5247, F-34095 Montpellier 5, France

[3] Univ Lyon 1, CNRS, Lab Chim Organ Glycochim 2, ICBMS,UMR 5246, F-69622 Villeurbanne, France

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2012年 / 77卷 / 17期

关键词：

CARBOHYDRATE-PROTEIN INTERACTIONS; DNA-DIRECTED IMMOBILIZATION; CLICK CHEMISTRY; GLYCOPEPTIDE DENDRIMERS; SOLID SUPPORT; HIGH-AFFINITY; OLIGONUCLEOTIDES; RECOGNITION; PHOSPHORAMIDITE; LIGANDS;

D O I：

10.1021/jo300826u

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Homo- and heterofunctionalized glycoclusters with galactose and/or fucose residues targeting both PA-IL and PA-IIL lectins of Pseudomonas aeruginosa were synthesized using "Click" chemistry and DNA chemistry. Their binding to lectins (separately or in a mixture) was studied using a DNA Directed Immobilization carbohydrate microarray. Homoglycoclusters bind selectively to their lectin while the heteroglycocluster binds simultaneously both lectins with a slight lower affinity.

引用

页码：7620 / 7626

页数：7

共 47 条

[41] The galactophilic lectin (PA-IL, gene LecA) from Pseudomonas aeruginosa.: Its binding requirements and the localization of lectin receptors in various mouse tissues
Kirkeby, Svend
Hansen, Axel K.
d'Apice, Anthony
Moe, Dennis
MICROBIAL PATHOGENESIS, 2006, 40 (05) : 191 - 197
[42] Comparison of the antimicrobial adhesion potential of human body fluid glycoconjugates using fucose-binding lectin (PA-IIL) of Pseudomonas aeruginosa and Ulex europaeus lectin (UEA-I)
Lerrer, B
Lesman-Movshovich, E
Gilboa-Garber, N
CURRENT MICROBIOLOGY, 2005, 51 (03) : 202 - 206
[43] Comparison of the Antimicrobial Adhesion Potential of Human Body Fluid Glycoconjugates Using Fucose-Binding Lectin (PA-IIL) of Pseudomonas aeruginosa and Ulex europaeus Lectin (UEA-I)
Batia Lerrer
Efrat Lesman-Movshovich
Nechama Gilboa-Garber
Current Microbiology, 2005, 51 : 202 - 206
[44] Binding Properties of the Galactose-detecting Lectin Pseudomonas aeruginosa Agglutinin (PA-IL) to Skeletal Muscle Fibres. Quantitative Precipitation and Precipitation Inhibition Assays
Svend Kirkeby
Poul E. Høyer
The Histochemical Journal, 1999, 31 : 485 - 493
[45] Molecular arrangement between multivalent glycocluster and Pseudomonas aeruginosa LecA (PA-IL) by atomic force microscopy: influence of the glycocluster concentration
Sicard, D.
Chevolot, Y.
Souteyrand, E.
Imberty, A.
Vidal, S.
Phaner-Goutorbe, M.
JOURNAL OF MOLECULAR RECOGNITION, 2013, 26 (12) : 694 - 699
[46] Binding properties of the galactose-detecting lectin Pseudomonas aeruginosa agglutinin (PA-IL) to skeletal muscle fibres.: Quantitative precipitation and precipitation inhibition assays
Kirkeby, S
Hoyer, PE
HISTOCHEMICAL JOURNAL, 1999, 31 (07): : 485 - 493
[47] Usage of the mannose-binding protein (lectin) Con A and the fucose-binding lectins UEA-I and PA-IIL for the study of glycoproteins from human milks and other body fluids
Gilboa-Garber, N
Lerrer, B
Lesman-Movshovich, E
FEBS JOURNAL, 2005, 272 : 547 - 548

← 1 2 3 4 5 →